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首页> 外文期刊>RSC Advances >Compartmentalized bioencapsulated liquefied 3D macro-construct by perfusion-based layer-by-layer technique
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Compartmentalized bioencapsulated liquefied 3D macro-construct by perfusion-based layer-by-layer technique

机译：通过基于灌流的逐层技术对分隔的生物封装液化3D宏结构进行分隔

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摘要

Self-supporting, millimeter length 3D constructs consisting of individualized liquefied compartments, were produced using cell encapsulated hydrogel beads as building blocks. A perfusion-based layer-by-layer approach was used that allowed bioencapsulated beads to assemble, pattern, hold and attach to produce non-liquefied 3D constructs with controlled shape, displaying the binding feature of a continuous nanometric multilayer coating. No binders or crosslinking strategies were used. The internal microenvironment of this 3D construct was modified from solid to liquefied state by chelation. MTS and live-dead assays showed enhanced L929 cell viability with liquefied 3D constructs, compared to non-liquefied ones. The proposed technique opens new prospects to create complex 3D polyelectrolyte based constructs for tissue engineering applications.
机译：使用细胞封装的水凝胶珠子作为构建基元，可以生成由独立的液化隔室组成的自支撑，毫米长的3D构建体。使用基于灌注的逐层方法，该方法允许生物封装的珠粒进行组装，构图，固定和附着，以产生形状受控的非液化3D构建体，从而显示出连续纳米多层涂层的结合特征。没有使用粘合剂或交联策略。通过螯合将该3D构建体的内部微环境从固态更改为液化状态。 MTS和活死分析显示，与非液化3D构建体相比，液化3D构建体增强了L929细胞的活力。所提出的技术为创建复杂的基于3D聚电解质的组织工程应用开辟了新的前景。

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