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首页> 外文期刊>RSC Advances >Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation
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Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation

机译：热响应性疏水共聚物刷改性多孔整体硅胶，用于高分辨率生物分离

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摘要

Thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-n-butyl methacrylate) [P(IPAAm-co-BMA)], brushes were prepared on porous monolithic silica rods through surface-initiated atom transfer radical polymerization (ATRP). The monolithic silica surface was modified with the ATRP-initiator [(chloromethyl)phenylethyl]trimethoxysilane (CPTMS) via silanization performed by flowing a CPTMS toluene solution into the monolithic silica rod column. ATRP was performed by flowing a reaction solution containing IPAAm and BMA monomers, and ATRP catalyst CuCl/CuCl2/tris[2-(N,N-dimethylamino)ethyl]amine in 2-propanol into the monolithic silica. Characterization by CHN elemental analysis, X-ray photoelectron spectroscopy (XPS), and gel permeation chromatography revealed that dense P(IPAAm-co-BMA) brushes were formed on the monolith surface. Chromatographic analysis using benzoic acids and insulin peptides indicated that the brush-modified monolithic silica was able to separate these analytes with high resolution and in short analysis times, owing to the effective interaction between the modified polymer brushes and the analyte. These results indicated that the present P(IPAAm-co-BMA) brush modified monolithic silica is an effective separation tool for analyzing bio-related molecules.
机译：通过表面引发的原子转移自由基聚合（ATRP）在多孔整体硅胶棒上制备了热响应性疏水共聚物聚（N-异丙基丙烯酰胺-甲基丙烯酸正丁酯）[P（IPAAm-co-BMA）]刷子。通过使CPTMS甲苯溶液流入整体硅胶棒柱中进行硅烷化，用ATRP引发剂[（氯甲基）苯基乙基]三甲氧基硅烷（CPTMS）改性整体硅胶表面。通过使包含IPAAm和BMA单体的反应溶液以及ATRP催化剂CuCl / CuCl2 / tris [2-（N，N-二甲基氨基）乙基]胺在2-丙醇中的反应溶液流入整体硅胶中，进行ATRP。通过CHN元素分析，X射线光电子能谱（XPS）和凝胶渗透色谱进行表征，发现在整体表面上形成了密集的P（IPAAm-co-BMA）刷子。使用苯甲酸和胰岛素肽的色谱分析表明，由于改性聚合物刷与分析物之间的有效相互作用，刷修饰的整体硅胶能够以高分辨率且在较短的分析时间内分离出这些分析物。这些结果表明，目前的P（IPAAm-co-BMA）刷改性的整体式二氧化硅是用于分析生物相关分子的有效分离工具。

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