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A comparative study on binding ability of three lanthanide ions with centrin using impedance method

机译：阻抗法比较三种镧系离子与中心蛋白结合能力的比较研究

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摘要

Centrin, an EF-hand calcium-binding protein, is involved in the formation of the microtubule organizing center (MTOC). The interactions between Gd3+, Eu3+, La3+ and the N-terminal domain of ciliate Euplotes octocarinatus centrin (N-EoCen) were investigated by electrochemical impedance spectroscopy (EIS) using potassium ferricyanide as a redox probe. Results show that an increase in the lanthanide ion (Ln(3+)) concentration resulted in a lower impedance magnitude in the Nyquist plots when the N-EoCenmodified glassy carbon electrode (N-EoCen-GC) was used. The titrations of N-EoCen with Ln(3+) confirm the complexation reactivity of Ln(3+) with N-EoCen and suggest that the Ln(2)-N-EoCen complex was formed. In contrast, the binding ability of Ca2+ to N-EoCen is far less than that of Ln(3+). The binding constants of the metal ions to N-EoCen are in the order of Gd3+ approximate to Eu3+ La3+ Ca2+. Compared with the resonance light scattering (RLS) method, the EIS method can not only distinguish the binding ability of different metal ions to N-EoCen, but can also distinguish clearly the different metal ion binding sites on N-EoCen.
机译：EF手钙结合蛋白Centrin参与了微管组织中心（MTOC）的形成。使用铁氰化钾作为氧化还原探针，通过电化学阻抗谱法（EIS）研究了Gd3 +，Eu3 +，La3 +与纤毛Euplotes octocarinatus centrin（N-EoCen）N末端结构域之间的相互作用。结果表明，当使用N-EoCen修饰的玻碳电极（N-EoCen-GC）时，镧系元素离子（Ln（3+））浓度的增加会导致奈奎斯特图中的阻抗值降低。用Ln（3+）滴定N-EoCen可以确认Ln（3+）与N-EoCen的络合反应性，并表明形成了Ln（2）-N-EoCen复合物。相反，Ca2 +与N-EoCen的结合能力远小于Ln（3+）。金属离子与N-EoCen的结合常数约为Gd3 +，近似于Eu3 + La3 + Ca2 +。与共振光散射（RLS）方法相比，EIS方法不仅可以区分不同金属离子与N-EoCen的结合能力，而且可以清楚地区分N-EoCen上不同的金属离子结合位点。

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