• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Preparation, characterization and analytical application of an electrochemical laccase biosensor towards low level determination of isoprenaline in human serum samples
【24h】

Preparation, characterization and analytical application of an electrochemical laccase biosensor towards low level determination of isoprenaline in human serum samples

机译：电化学漆酶生物传感器的制备，表征和分析在低水平测定人血清样品中异丙肾上腺素的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel electrochemical biosensor has been developed based on the immobilization of multiwalled carbon nanotubes (MWCNT) on to a glassy carbon electrode (GCE) and subsequent casting of silica sol-gel (SiSG) entrapped laccase (Lac) enzyme on to the MWCNT/GCE. The catalytic activity of laccase biosensor was found to be good enough for sensitive determination of isoprenaline (ISP) with the aid of voltammetric techniques and we have also demonstrated the detailed electrochemical redox mechanism of ISP. From the effect of the pH, we have optimized the optimum pH as 6.5, and from effect of scan rate we have evaluated the kinetic parameters, heterogeneous rate constant, charge transfer coefficient and diffusion coefficient values. Furthermore the limit of detection (LOD) and limit of quantification (LOQ) values were found to be 1.8 x 10(-7) M and 6.0 x 10(-7) M, respectively. The simultaneous determination of ISP in the presence of uric acid (UA) and ascorbic acid (AA) was successfully carried out. The surface nature of the biosensor was characterized by using electrochemical impedance spectroscopy. Finally the validation of the proposed method was verified by the recovery of injection (ISP) in serum samples and their recoveries were found to be in a satisfactory range. The proposed method was found to have good repeatability, reproducibility and stability with low relative standard deviation (RSD) values.
机译：基于将多壁碳纳米管（MWCNT）固定在玻璃碳电极（GCE）上，然后将硅胶溶胶（SiSG）包裹的漆酶（Lac）酶铸塑到MWCNT / GCE上，开发了一种新型的电化学生物传感器。发现漆酶生物传感器的催化活性足以借助伏安法灵敏地测定异丙肾上腺素（ISP），我们还证明了ISP的详细电化学氧化还原机理。根据pH的影响，我们已将最佳pH优化为6.5，并且根据扫描速率的影响，我们评估了动力学参数，异质速率常数，电荷转移系数和扩散系数值。此外，发现检测限（LOD）和定量限（LOQ）值分别为1.8 x 10（-7）M和6.0 x 10（-7）M。成功地在尿酸（UA）和抗坏血酸（AA）的存在下同时测定ISP。生物传感器的表面性质通过使用电化学阻抗谱来表征。最后，通过血清样品中进样的回收率（ISP）验证了所提方法的有效性，发现其回收率在令人满意的范围内。发现该方法具有良好的重复性，重现性和稳定性，并且相对标准偏差（RSD）值较低。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号