Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions

Gupta Bhupender S.; Taha Mohamed; Lee Ming-Jer

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Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions

机译：α-胰凝乳蛋白酶与生物缓冲液，TRIS，TES，TAPS和TAPSO在水溶液中的超活性

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Biological buffers are always considered as non-toxic, biocompatible and green compounds. Therefore, we analyzed the catalytic activity of a commercial enzyme alpha-chymotrypsin (alpha-CT) in aqueous solutions of some common biological buffers (TRIS, TES, TAPS, and TAPSO) at pH 8 and T = 25 degrees C. It is found that the increase of the buffer concentration enhanced the catalytic activity of enzyme alpha-CT, and the tendency follows the order of TRIS > TES > TAPS > TAPSO. Especially in the presence of TRIS, the catalytic activity enhanced 5.5 fold.

机译：生物缓冲液一直被认为是无毒的，生物相容的绿色化合物。因此，我们分析了商业酶α-胰凝乳蛋白酶（α-CT）在一些常见的生物缓冲液（TRIS，TES，TAPS和TAPSO）在pH 8和T = 25摄氏度的水溶液中的催化活性。缓冲液浓度的增加增强了α-CT酶的催化活性，其趋势遵循TRIS> TES> TAPS> TAPSO的顺序。特别是在TRIS存在下，催化活性提高了5.5倍。

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《RSC Advances》 |2014年第93期|共6页
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Gupta Bhupender S.; Taha Mohamed; Lee Ming-Jer;
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1. Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions [J] . Gupta Bhupender S., Taha Mohamed, Lee Ming-Jer RSC Advances . 2014,第93期

机译：α-胰凝乳蛋白酶与生物缓冲液，TRIS，TES，TAPS和TAPSO在水溶液中的超活性
2. Complexation of M-(buffer)(x)-(OH)(y) systems involving divalent ions (cobalt or nickel) and zwitterionic biological buffers (AMPSO, DIPSO, TAPS and TAPSO) in aqueous solution [J] . Machado CMM, Gameiro P, Soares HMVM Journal of Solution Chemistry . 2008,第5期

机译：M-（缓冲液）（x）-（OH）（y）系统与二价离子（钴或镍）和两性离子生物缓冲液（AMPSO，DIPSO，TAPS和TAPSO）的络合
3. Buffer interactions: Solubilities and transfer free energies of TRIS, TAPS, TAPSO, and TABS from water to aqueous ethanol solutions [J] . Taha M, Lee MJ Fluid Phase Equilibria . 2010,第2期

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4. Biodegradation Study of Calcium Metaphosphate Coated by Sol-Gel Method in the SBF and Tris-Buffer Solution [C] . Sunho Oh, Shin Yoon Kim, Changkook You International symposium on ceramics in medicine . 2001

机译：SBF和Tris缓冲溶液中溶胶法涂覆杂化磷酸钙的生物降解研究
5. ThepH buffering effect and charging behavior of oxides in aqueous solution [D] . Park, Jaehyeon. 1995

机译：水溶液中氧化物的pH缓冲作用和充电行为
6. Models for enzyme superactivity in aqueous solutions of surfactants. [O] . P Viparelli, F Alfani, M Cantarella 1999

机译：表面活性剂水溶液中酶超活性的模型。
7. Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions [O] . Gupta, Bhupender S., Taha, Mohamed, Lee, Ming-Jer 2014

机译：α-胰凝乳蛋白酶与生物缓冲液，TRIS，TES，TAPS和TAPSO在水溶液中的超活性

Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions

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