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首页> 外文期刊>RSC Advances >Magnetic mesoporous silica nanoparticles for CpG delivery to enhance cytokine induction via toll-like receptor 9
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Magnetic mesoporous silica nanoparticles for CpG delivery to enhance cytokine induction via toll-like receptor 9

机译：磁性介孔二氧化硅纳米颗粒，用于CpG递送，可通过通行费样受体9增强细胞因子的诱导作用

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摘要

We developed a potential cytosine-phosphate-guanosine oligodeoxynucleotide (CpG ODN) delivery system based on magnetic mesoporous silica (MMS) nanoparticles by binding of CpG ODN onto aminated MMS (MMS-NH2) nanoparticles to form CpG/MMS-NH2 complexes for toll-like receptor 9 (TLR9)-mediated induction of cytokines. Magnetization, serum stability, in vitro cytotoxicity, cellular uptake, and interleukin-6 (IL-6) induction of CpG/MMS-NH2 complexes were evaluated. The results showed that MMS nanoparticles exhibited superparamagnetic behavior with a saturation magnetization of 6.5 emu g(-1). Also, MMS-NH2 nanoparticles had no cytotoxicity to Raw 264.7 cells, and CpG/MMS-NH2 complexes enhanced the serum stability of CpG ODN and could be localized in the endolysosomes after endocytosis by cells. Importantly, CpG/MMS-NH2 complexes significantly enhanced the TLR9-mediated IL-6 induction compared to free CpG ODN. Therefore, CpG/MMS-NH2 complexes could exhibit magnetic targeted delivery and significantly enhance the TLR9-mediated cytokine induction for stimulating immune responses.
机译：我们通过将CpG ODN结合到胺化MMS（MMS-NH2）纳米颗粒上形成CpG / MMS-NH2复合物以形成收费的基础，我们开发了基于磁性介孔二氧化硅（MMS）纳米颗粒的潜在的胞嘧啶-磷酸-鸟苷寡聚脱氧核苷酸（CpG ODN）递送系统。像受体9（TLR9）介导的细胞因子诱导。评估了CpG / MMS-NH2复合物的磁化，血清稳定性，体外细胞毒性，细胞摄取和白介素6（IL-6）诱导。结果表明，MMS纳米粒子表现出超顺磁行为，饱和磁化强度为6.5 emu g（-1）。而且，MMS-NH2纳米颗粒对Raw 264.7细胞没有细胞毒性，CpG / MMS-NH2复合物增强了CpG ODN的血清稳定性，并且在细胞被细胞内吞后可以定位在溶酶体中。重要的是，与游离CpG ODN相比，CpG / MMS-NH2复合物显着增强了TLR9介导的IL-6诱导。因此，CpG / MMS-NH2复合物可以表现出磁性靶向递送，并显着增强TLR9介导的细胞因子诱导，以刺激免疫反应。

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