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首页> 外文期刊>RSC Advances >Mesenchymal stem cell response to UV-photofunctionalized TiO2 coated CoCrMo
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Mesenchymal stem cell response to UV-photofunctionalized TiO2 coated CoCrMo

机译：间充质干细胞对紫外光功能化TiO2涂层CoCrMo的反应

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摘要

Ultraviolet (UV) photofunctionalization has been shown to be highly effective at improving the osteoconductivity of titanium and TiO2 coated materials. We aimed to assess whether the bioactivity of TiO2 coated cobalt chromium molybdenum (CoCrMo) could be enhanced by UV photofunctionalization of the surface TiO2 layer. Using atmospheric pressure chemical vapour deposition (APCVD) a thin layer of anatase TiO2 was deposited onto smooth CoCrMo discs (referred to as CCMT). Human mesenchymal stem cells (MSCs) were cultured onto CCMT substrates which had been treated with UV light for 24 hours and identical substrates which had not undergone UV treatment. UV treated CCMT promoted a superior cell response in the form of enhancing markers of cell adhesion. This included stimulating the development of larger cells with increased levels of the adhesion protein vinculin and cytoskeletal protein f-actin (p < 0.05). In addition, MSCs were shown to have superior retention to UV treated CCMT after 3 and 24 hours (p < 0.05). Other cellular processes including proliferation, attachment, migration and differentiation were not affected by UV photofunctionalization. Despite this, the enhancement in cellular adhesion alone should result in an improvement in MSC retention to implant surfaces following surgery, and as a consequence, increase MSC resistance to dislodgement from external forces such as blood flow and micro motion.
机译：紫外线（UV）光功能化已被证明在改善钛和TiO2涂层材料的骨传导性方面非常有效。我们旨在评估是否可以通过表面TiO2层的UV光官能化来增强TiO2涂层的钴铬钼（CoCrMo）的生物活性。使用大气压化学气相沉积（APCVD），将锐钛矿型TiO2薄层沉积到光滑的CoCrMo圆盘（称为CCMT）上。将人间充质干细胞（MSC）培养在已用UV光处理24小时的CCMT底物和未进行UV处理的相同底物上。紫外线处理的CCMT以增强细胞粘附标记的形式促进了优异的细胞反应。这包括以粘附蛋白纽蛋白和细胞骨架蛋白f-肌动蛋白水平升高来刺激较大细胞的发育（p <0.05）。此外，在3和24小时后，MSCs表现出优于UV处理的CCMT（p <0.05）。其他细胞过程包括增殖，附着，迁移和分化不受紫外线光功能化的影响。尽管如此，仅细胞粘附的增强将导致手术后MSC对植入物表面的固着性改善，结果，增加MSC抵抗外力（如血流和微动）脱位的抵抗力。

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