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A dual-mode luminescent probe composed of co-assembled down-conversion CdTe and up-conversion NaYF4:Yb, Tm(Er) nanoparticles

机译:由共组装的下转换CdTe和上转换NaYF4:Yb,Tm(Er)纳米粒子组成的双模发光探针

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摘要

Multiplex luminescent probes with good water-dispersibility are basic materials in current biological research as labels and sensors. In this work, we demonstrate a facile approach to fabricate water-dispersible dual-mode luminescent probes by co-assembling down-conversion CdTe nanoparticles (NPs) and up-conversion NaYF4:Yb, Tm(Er) NPs. 3-Mercaptopropionic acid (MPA)-capped CdTe NPs are water soluble, while oleic acid (OA)-capped NaYF4: Yb, Tm(Er) NPs are dispersed in chloroform. There also exists an excess of Cd-MPA complex in the CdTe aqueous solution. After mixing CdTe and NaYF4:Yb, Tm(Er), the MPA on CdTe NPs and Cd-MPA complexes is capable of partially replacing the OA on NaYF4:Yb, Tm(Er) NPs, thus transferring NaYF4:Yb, Tm(Er) NPs to water and forming CdTeNaYF4:Yb, Tm(Er) hybrid NPs. The hybrid NPs combine the down-conversion emission of CdTe and the up-conversion emission of NaYF4:Yb, Tm(Er), which can be excited both by 400 and 980 nm irradiation and exhibit dual-mode emission. Both Forster resonance energy transfer from NaYF4:Yb, Tm(Er) to CdTe and photon re-absorption are observed as the hybrid NPs are excited by 980 nm irradiation.
机译:具有良好水分散性的多重发光探针是当前生物学研究中用作标记和传感器的基本材料。在这项工作中,我们演示了通过组装下转换CdTe纳米粒子(NP)和上转换NaYF4:Yb,Tm(Er)NP来制造水分散性双模发光探针的简便方法。 3-巯基丙酸(MPA)封端的CdTe NPs是水溶性的,而油酸(OA)封端的NaYF4:Yb,Tm(Er)NPs分散在氯仿中。 CdTe水溶液中也存在过量的Cd-MPA络合物。将CdTe和NaYF4:Yb,Tm(Er)混合后,CdTe NPs和Cd-MPA复合物上的MPA能够部分替代NaYF4:Yb,Tm(Er)NPs上的OA,从而转移NaYF4:Yb,Tm(Er )NPs遇水并形成CdTeNaYF4:Yb,Tm(Er)杂化NPs。混合NP结合了CdTe的下转换发射和NaYF4:Yb,Tm(Er)的上转换发射,NaYF4:Yb,Tm(Er)可以被400和980 nm照射激发,并呈现双模发射。从980 nm辐照激发杂化NPs可以观察到从NaYF4:Yb,Tm(Er)到CdTe的Forster共振能量转移和光子重吸收。

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