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Time-resolved confocal microscopy using lanthanide centred near-IR emission

机译:使用镧系元素为中心的近红外发射的时间分辨共聚焦显微镜

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摘要

A method to uniquely identify signals originating from probes with different emission decay times in luminescence imaging has been developed. By using scanning confocal microscopy in combination with time-correlated single photon counting (TCSPC), Photon Arrival Time Imaging (PArTI) has been realised through off-line plotting of images using the photon arrival times. PArTI is the time-equivalent to spectrally resolved imaging, replacing the energy axis with a photon arrival time axis. Here, lanthanide probes were used to demonstrate the key advantages of themethod. PArTI uses TCSPC data, involves no fitting, uses a single pulsed laser line for multicolour imaging, and can be used with a 100 millisecond dwell time per pixel.
机译:已经开发了一种在发光成像中唯一地识别源自具有不同发射衰减时间的探针的信号的方法。通过将扫描共聚焦显微镜与时间相关的单光子计数(TCSPC)结合使用,可以通过使用光子到达时间对图像进行离线绘图来实现光子到达时间成像(PArTI)。 PArTI是时间等效于光谱分辨成像,用光子到达时间轴代替了能量轴。在这里,镧系元素探针被用来证明该方法的关键优势。 PArTI使用TCSPC数据,不涉及拟合,使用单脉冲激光线进行彩色成像,并且每个像素的驻留时间为100毫秒。

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