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Ultra-sensitive detection of heparin via aPTT using plastic antibodies on QCM-D platform

机译：在QCM-D平台上使用塑料抗体通过aPTT超灵敏检测肝素

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One of the challenges faced by today's clinics is unavailability of practical, precise and accurate determination of the most commonly used anti-coagulant 'heparin' in human blood/plasma in surgery. This is the first report of the application of a heparin imprinted thin film on a Quartz Crystal Microbalance with Dissipation (QCM-D) platform. Based on a novel, straight-forward and robust imprinting approach, a lowest volume consumption of 2 mu L of real sample (of human plasma) has been demonstrated. This is a leap for launching spot testing for heparin sensing in laboratory practice. This approach could shorten the coagulation times to 48% and 45% for clinically important sensing range of heparin doses of 0.75 and 0.50 IU mL(-1) respectively and could improve precision 3-fold (n = 20 samples) compared to that of today's 'gold standard'. Activated partial thromboplastin time (aPTT) on the plastic antibodies based QCM-D platform for plasma with doses of heparin 0.75 IU mL(-1) and 0.50 IU mL(-1) yielded 6.5% and 5% lower %RSD respectively as compared to that of the 'gold standard'. The present studies could provide a launch pad to laboratory methods for ultra-refining of point of care (POC) settings in the perspectives of coagulation time shortening, precision of the method and sample volume consumption.
机译：当今诊所面临的挑战之一是无法获得实用，精确和准确的手术中人类血液/血浆中最常用的抗凝剂“肝素”的测定方法。这是在具有消散作用的石英晶体微天平（QCM-D）平台上应用肝素印迹薄膜的第一份报告。基于一种新颖，直接而强大的压印方法，已证明最低消耗量为2μL（人类血浆）的真实样品。这是在实验室实践中启动肝素检测现场测试的飞跃。对于肝素剂量为0.75和0.50 IU mL（-1）的临床上重要的感测范围，该方法可以将凝血时间分别缩短至48％和45％，并且与当今的相比，可以将精度提高3倍（n = 20个样品）。 '黄金标准'。与基于0.75 IU mL（-1）和0.50 IU mL（-1）肝素的血浆基于塑料抗体的QCM-D平台上激活的部分凝血活酶时间（aPTT）相比，％RSD分别降低了6.5％和5％ “黄金标准”。本研究可以为缩短凝结时间，方法的精确度和样品量消耗的角度提供超细的护理点（POC）设置的实验室方法的启动平台。

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