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Magnetic-EpCAM nanoprobe as a new platform for efficient targeting, isolating and imaging hepatocellular carcinoma

机译：Magnetic-EpCAM纳米探针作为有效靶向，分离和成像肝细胞癌的新平台

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摘要

Herein, magnetic-EpCAM nanoparticle (EpCAM-MNP) was developed and exploited as nanoprobe for targeting, isolating and imaging hepatocellular carcinoma. The nanoprobe was composed of two major components including aminosilane-coated iron oxide nanoparticles and DNA-based EpCAM aptamers. Extensive studies were carried out to investigate its physico-chemical properties, magnetic relaxivity, as well as biocompatibility. The results indicated that the small size of the nanoprobe (HD < 100 nm) had high R-2 relaxivity with good biocompatibility. Study on cellular accumulation and cellular uptake demonstrated high accumulation of EpCAM-MNP that was internalized via receptor-mediated endocytosis, as evidenced by TEM analysis. By using a magnetic-activated cell sorting (MACS) approach, the majority (similar to 96%) of the HepG2 cells were isolated, indicating the feasibility of our nanoprobe for isolating EpCAM-positive cells in clinical application. Because of the high intracellular accumulation and the high R-2 relaxivity of EpCAM-MNP, it can be used for quantitative MRI detection of cancer cells with high sensitivity.
机译：在本文中，磁性-EpCAM纳米颗粒（EpCAM-MNP）被开发出来，并被用作靶向，分离和成像肝细胞癌的纳米探针。纳米探针由两个主要成分组成，包括氨基硅烷涂层的氧化铁纳米粒子和基于DNA的EpCAM适体。进行了广泛的研究以研究其理化性质，磁弛豫性以及生物相容性。结果表明，小尺寸的纳米探针（HD <100 nm）具有较高的R-2弛豫性和良好的生物相容性。细胞积累和细胞摄取的研究表明，EpCAM-MNP的高积累是通过受体介导的内吞作用而被内在化的，这通过TEM分析得以证明。通过使用磁激活细胞分选（MACS）方法，分离了大多数（约96％）的HepG2细胞，这表明我们的纳米探针在临床应用中分离EpCAM阳性细胞的可行性。由于EpCAM-MNP具有较高的细胞内蓄积性和R-2弛豫性，因此可用于以高灵敏度对癌细胞进行MRI定量检测。

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