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首页> 外文期刊>RSC Advances >Identification of miRNAs and their targets in transgenic Brassica napus and its acceptor (Westar) by high-throughput sequencing and degradome analysis
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Identification of miRNAs and their targets in transgenic Brassica napus and its acceptor (Westar) by high-throughput sequencing and degradome analysis

机译：通过高通量测序和降解组分析鉴定转基因甘蓝型油菜及其受体（Westar）中的miRNA及其靶标

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摘要

MicroRNAs (miRNAs) are a class of noncoding small RNAs (sRNAs) that play many roles in plant growth, development, and the stress response. Recently, the Brassica napus (B. napus) genome was reported, which allows the genome-wide identification of miRNAs and their targets in B. napus cv. Westar and its transgenic cultivar. Thus, two sRNAs libraries and two degradome libraries of the transgenic B. napus (TG) and its acceptor (B. napus cv. Westar, WA) were constructed. Following high-throughput sequencing and miRNA identification, 139 unique conserved and 305 unique novel miRNA sequences were identified in the two sRNA libraries, and through degradome analysis, 540 targets corresponding to 167 unique miRNA sequences were identified in the two degradome libraries. 11 differentially expressed unique miRNA sequences were verified by quantitative real-time PCR (qRT-PCR), and the results nearly agreed with the high-throughput sequencing data. The present study increases the number of miRNAs identified in B. napus and suggests that there are possible differences in the miRNA expression between transgenic B. napus and its acceptor.
机译：MicroRNA（miRNA）是一类非编码小RNA（sRNA），在植物生长，发育和胁迫响应中起着许多作用。最近，报道了甘蓝型油菜（B. napus）基因组，该基因组可以在全基因组范围内鉴定miRNA及其在油菜中的靶标。 Westar及其转基因品种。因此，构建了转基因甘蓝型油菜（TG）及其受体（甘蓝型油菜种Westar，WA）的两个sRNA文库和两个降解组文库。经过高通量测序和miRNA鉴定后，在两个sRNA文库中鉴定了139个独特的保守miRNA序列和305个独特的新颖miRNA序列，并且通过降解组分析，在两个降解基因组文库中鉴定了对应于167个独特miRNA序列的540个靶标。通过定量实时PCR（qRT-PCR）验证了11个差异表达的独特miRNA序列，其结果与高通量测序数据几乎吻合。本研究增加了在甘蓝型油菜中鉴定出的miRNA的数量，并表明转基因甘蓝型油菜与其受体之间的miRNA表达可能存在差异。

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