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首页> 外文期刊>RSC Advances >Green synthesis, characterization and anticancer activity of luminescent gold nanoparticles capped with apo-alpha-lactalbumin
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Green synthesis, characterization and anticancer activity of luminescent gold nanoparticles capped with apo-alpha-lactalbumin

机译：载有脱辅基-α-乳清蛋白的发光金纳米颗粒的绿色合成，表征和抗癌活性

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摘要

A green synthesis was developed in order to prepare protein coated gold nanoparticles using a metal free, alpha-helical protein, i.e., apo-alpha-LA, that acts as both the reducing as well as stabilizing agent to result in Au-0 nanoparticles from Au3+ which are luminescent and hence can be used for biological imaging, including of cells. The nanoparticles, apo-alpha-LA-AuNPs, thus synthesized were characterized by multiple techniques, such as, analytical, spectral, microscopy and light scattering, in order to support the presence of NPs in terms of their size and shape, involvement of Au-0 and the protein encapsulation and its structural changes upon coating. The 10-16 nm sized apo-alpha-LA-AuNPs were shown to be non-toxic to healthy cells as studied using normal mouse fibroblast cells (L929). Having found that these NPs are biocompatible and possess structurally altered apo-alpha-LA protein, the luminescent apo-alpha-LA-AuNPs were demonstrated to have cytotoxicity towards cancer cells as studied by cell viability tests as well as fluorescence microscopy. While these NPs kill similar to 75% of MCF-7 cells, at the same concentration these are capable of killing only similar to 30% of HeLa cells, thus exhibiting cell dependency. The present study clearly demonstrates the advantage of the luminescent apo-alpha-LA-AuNPs in their attack of cancer cells in general and selective killing of breast cancer cells in particular. Thus coating AuNPs with the protein apo-alpha-LA enhanced their anticancer activity several fold.
机译：为了使用无金属的α-螺旋蛋白（即载脂蛋白-α-LA）制备还原蛋白和稳定剂以产生Au-0纳米颗粒，开发了一种绿色合成方法，以制备蛋白包被的金纳米颗粒发光的Au3 +，因此可用于包括细胞在内的生物成像。如此合成的纳米颗粒，载脂蛋白-α-LA-AuNPs通过多种技术进行表征，例如分析，光谱，显微镜和光散射，以支持NPs的大小和形状，Au的参与-0和蛋白质包被及其包被后的结构变化。如使用正常小鼠成纤维细胞（L929）研究的，显示10-16 nm大小的apo-alpha-LA-AuNP对健康细胞无毒。已经发现这些NP具有生物相容性并具有结构改变的载脂蛋白α-LA蛋白，通过细胞生存力测试和荧光显微镜研究证明，发光的载脂蛋白α-LA-AuNP对癌细胞具有细胞毒性。尽管这些NP杀死了约75％的MCF-7细胞，但在相同浓度下，它们只能杀死约30％的HeLa细胞，从而表现出细胞依赖性。本研究清楚地证明了发光的载脂蛋白-α-LA-AuNPs在其对癌细胞的攻击中的优势，特别是对乳腺癌细胞的选择性杀伤。因此，用蛋白载脂蛋白-α-LA包被AuNPs可以将其抗癌活性提高数倍。

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