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A facile label-free colorimetric aptasensor for ricin based on the peroxidase-like activity of gold nanoparticles

机译：基于金纳米颗粒过氧化物酶样活性的蓖麻毒蛋白的简便无标记比色适体传感器

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摘要

AuNPs possess peroxidase-like activity that could catalyze 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, leading to color change of the solution. Herein we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of ricin by using AuNPs. It is shown that the peroxidase-like activity of AuNPs can be improved by surface activation with target-specific aptamers. However, with target molecules, the aptamer is desorbed from the AuNPs' surface, resulting in a decrease of the catalytic abilities of AuNPs. The color change of the solution was relevant to the target concentration, and this can be judged by the naked eye and monitored by UV-vis spectrometry. The linear range for the current analytical system was from 0.05 nM to 10 nM. The corresponding limit of detection (LOD) was 0.05 nM. Some other proteins such as thrombin (Th), glucose oxidase (GOx), and bovine albumin (BSA) all had a negligible effect on the determination of ricin. What is more, several practical samples spiked with ricin were analyzed using the proposed method with excellent recoveries. This colorimetric aptasensor is superior to the other conventional methods owing to its simplicity, low cost, and high sensitivity.
机译：AuNPs具有过氧化物酶样活性，可以在H2O2存在下催化3,3,5,5-四甲基联苯胺（TMB），导致溶液变色。在这里，我们提出了一种简单而灵敏的比色适体传感器，用于通过使用AuNPs进行蓖麻毒素的定量分析。结果表明，可以通过用靶标特异性适体进行表面活化来改善AuNP的过氧化物酶样活性。然而，对于靶分子，适体从AuNPs的表面解吸，导致AuNPs的催化能力降低。溶液的颜色变化与目标浓度有关，可以通过肉眼判断并通过紫外可见光谱法进行监测。当前分析系统的线性范围为0.05 nM至10 nM。相应的检出限（LOD）为0.05 nM。其他一些蛋白质，例如凝血酶（Th），葡萄糖氧化酶（GOx）和牛白蛋白（BSA）对蓖麻毒素的测定影响可忽略不计。此外，使用提出的方法分析了一些实际的加有蓖麻毒素的样品，回收率极高。该比色适体传感器由于其简单，低成本和高灵敏度而优于其他常规方法。

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