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首页> 外文期刊>RSC Advances >Dendritic Pt@Au nanowires as nanocarriers and signal enhancers for sensitive electrochemical detection of carcinoembryonic antigen
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Dendritic Pt@Au nanowires as nanocarriers and signal enhancers for sensitive electrochemical detection of carcinoembryonic antigen

机译：树突状Pt @ Au纳米线作为纳米载体和信号增强剂，用于癌胚抗原的敏感电化学检测

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摘要

Highly sensitive detection of carcinoembryonic antigen (CEA) is very important in clinical diagnosis and treatment assessment of cancers. In this work, we proposed a sensitive and selective electrochemical aptasensor for CEA detection using dendritic Pt@Au nanowires (Pt@AuNWs) as nanocarriers and electrocatalysts. With many advantages such as large specific surface area, good conductivity, excellent electrocatalytic activity and high stability, dendritic Pt@AuNWs were first employed as nanocarriers for immobilizing abundant thiol-terminated CEA aptamer 2 (CEAapt2) and redox-active toluidine blue (Tb), resulting in the formation of AuNWs-CEAapt2-Tb bioconjugate. In the presence of CEA, the proposed bioconjugate was captured onto the electrode surface through "sandwich" tactics. The electrochemical response was then triggered and further enhanced due to the favorable catalysis capacity of dendritic Pt@AuNWs with peroxidase mimic activity for the reduction of H2O2 added into the electrolytic cell, from which an improved sensitivity benefited and was successfully achieved. Under the optimal experimental conditions, the proposed aptasensor exhibited a linear response to CEA in the range of 0.001 ng mL(-1) to 80 ng mL(-1) and the limit of detection (LOD) is 0.31 pg mL(-1). Moreover, the aptasensor exhibited good selectivity, stability and reproducibility, which indicated its potential applications in clinical diagnostics.
机译：癌胚抗原（CEA）的高灵敏度检测在癌症的临床诊断和治疗评估中非常重要。在这项工作中，我们提出了一种灵敏的选择性电化学适体传感器，用于使用树突状Pt @ Au纳米线（Pt @ AuNWs）作为纳米载体和电催化剂的CEA检测。具有大比表面积，良好的电导率，优异的电催化活性和高稳定性等优点，首先将树状Pt @ AuNWs用作纳米载体，以固定大量巯基封端的CEA适体2（CEAapt2）和氧化还原活性甲苯胺蓝（Tb） ，导致形成AuNWs-CEAapt2-Tb生物缀合物。在CEA的存在下，通过“三明治”策略将提出的生物缀合物捕获到电极表面上。然后，由于具有过氧化物酶模拟活性的树突状Pt @ AuNWs具有良好的催化能力，可减少电化学反应，并进一步增强了电化学反应，从而减少了添加到电解池中的H2O2，从中受益并成功实现了改进。在最佳实验条件下，拟议的适体传感器对CEA表现出线性响应，范围为0.001 ng mL（-1）至80 ng mL（-1），检出限（LOD）为0.31 pg mL（-1） 。此外，适体传感器表现出良好的选择性，稳定性和可重复性，表明其在临床诊断中的潜在应用。

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