• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Portable plastic syringe as a self-actuated pump for long-distance uniform delivery of liquid inside a microchannel and its application for flow-through polymerase chain reaction on chip
【24h】

Portable plastic syringe as a self-actuated pump for long-distance uniform delivery of liquid inside a microchannel and its application for flow-through polymerase chain reaction on chip

机译：便携式塑料注射器作为一种自动驱动的泵，可在微通道内实现液体的长距离均匀输送及其在芯片上流通式聚合酶链反应中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A portable plastic syringe was used as a self-actuated pump for uniform delivery of liquid inside a microchannel over a distance of more than 2 meters at a controllable flow rate and without utilizing external electrical power and bulky pumping apparatus. The reliability of the plastic syringe as a potential self-actuator was investigated by performing a flow-through polymerase chain reaction (PCR) on a microdevice fabricated using poly(methylmethacrylate) (PMMA). Liquid flowed at a uniform rate inside the PMMA microchannel in a highly controllable manner even under high-temperature conditions and without the generation of bubbles, and the flow rate was readily adjusted as necessary by varying the operation parameters such as the length of the outlet silicone tube, channel dimension, and initial syringe pressure. A 230 bp plasmid vector obtained from E. coli and a D1S80 locus obtained from a human genomic DNA were successfully amplified on a PMMA microdevice equipped with the disposable plastic syringe as a self-actuated micropump.
机译：便携式塑料注射器用作自驱动泵，用于在可控流速下在超过2米的距离内均匀地在微通道内均匀地输送液体，而无需使用外部电源和笨重的泵送设备。通过在使用聚甲基丙烯酸甲酯（PMMA）制成的微型设备上进行流通式聚合酶链反应（PCR），研究了塑料注射器作为潜在自我致动器的可靠性。即使在高温条件下，液体也能以高度可控的方式以均匀的速率在PMMA微通道内流动，并且不会产生气泡，并且可以通过改变操作参数（例如出口硅胶的长度）轻松地根据需要调节流量管，通道尺寸和初始注射器压力。在配备有一次性塑料注射器作为自驱动微型泵的PMMA微型设备上成功扩增了从大肠杆菌获得的230 bp质粒载体和从人基因组DNA获得的D1S80基因座。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号