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首页> 外文期刊>RSC Advances >Sensitive detection of C. parvum using near infrared emitting Ag2S@silica core-shell nanospheres
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Sensitive detection of C. parvum using near infrared emitting Ag2S@silica core-shell nanospheres

机译：使用近红外发射Ag2S @二氧化硅核-壳纳米球灵敏检测小球藻

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摘要

An optical immunosensor was developed using anti-oocysts McAb immobilized near-infrared (NIR) emitting Ag2S@silica core-shell nanospheres for the detection of C. parvum in water. The formation of the core Ag2S and the SiO2 shell over the core was confirmed by X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM). The optical properties of the nanostructures were determined by UV-Vis spectroscopy and photoluminescence (PL) studies. The core-shell Ag2S@silica nanospheres exhibited an intense emission peak at 896 nm which falls in the biological window. TEM images confirm the presence of a uniform core-shell structure, with a Ag2S core with an average size of 80 nm, and a silica shell with a thickness of 40-50 nm. The C. parvum antibody anti-oocysts McAb immobilized Ag2S@silica nanoparticles were used as detector probes and these biosensors exhibited excellent analytical performance toward the detection of C. parvum, with detection limits of 10 oocysts per mL with a minimal assay period.
机译：开发了一种光学免疫传感器，使用抗卵囊菌单克隆抗体固定化的近红外（NIR）发射Ag2S @二氧化硅核-壳纳米球，用于检测水中的小球藻。 X射线衍射（XRD）和高分辨率透射电子显微镜（HRTEM）证实了Ag2S核和SiO2壳在核上的形成。纳米结构的光学性质通过紫外-可见光谱和光致发光（PL）研究确定。核壳型Ag2S @二氧化硅纳米球在896 nm处显示出强烈的发射峰，该峰落在生物窗口中。 TEM图像证实存在均匀的核-壳结构，其中Ag2S核的平均尺寸为80 nm，二氧化硅壳的厚度为40-50 nm。微小隐孢子虫抗体抗卵囊菌固定化的Ag2S @二氧化硅纳米颗粒用作检测探针，这些生物传感器对微小隐孢子虫的检测表现出出色的分析性能，检测限为每毫升10个卵囊，检测周期最短。

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