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Design and development of papain-urea loaded PVA nanofibers for wound debridement

机译：木瓜尿素载PVA纳米纤维伤口清创术的设计与开发

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摘要

Devitalized tissues present in a wound bed serve as a reservoir for bacterial growth and contain elevated levels of inflammatory mediators that promote chronic inflammation and impair cellular migration necessary for wound repair. Effective wound cleansing and debridement are essential for granulation and re-epithelization. Among various debridement methods, enzymatic debridement is a highly selective method that uses naturally occurring proteolytic enzymes. Papain combined with urea has been widely used to remove necrotic/devitalized tissues. Our approach is to encapsulate papain and urea in PVA nanofibers to bring out sustained release to enable breakdown of fibrinous material in necrotic tissue and enhance wound healing. Physico-chemical characterization of nanofibers depicted the enzyme interaction with the polymer and also confirmed that the enzyme was evenly distributed in the nanofibers in an amorphous state. Fluorescence spectroscopy confirmed that the structural integrity of the enzyme was maintained after encapsulation. The results of antibacterial activity along with cell compatibility assays confirm the structural and functional integrity of the enzyme preparation along with the biocompatibility of the electrospun nanofiber and thereby provide more suitability as a dressing for wound debridement.
机译：伤口床中存在的失活组织充当细菌生长的储存库，并含有升高水平的炎症介质，从而促进慢性炎症并损害伤口修复所必需的细胞迁移。有效的伤口清洁和清创术对于肉芽形成和重新上皮化至关重要。在多种清创方法中，酶促清创是使用天然蛋白水解酶的高度选择性方法。木瓜蛋白酶与尿素的结合已被广泛用于去除坏死/失活的组织。我们的方法是将木瓜蛋白酶和尿素包裹在PVA纳米纤维中，以实现持续释放，从而使坏死组织中的纤维蛋白降解并增强伤口愈合。纳米纤维的理化特性描述了酶与聚合物的相互作用，也证实了酶以无定形状态均匀地分布在纳米纤维中。荧光光谱证实了包封后酶的结构完整性得以保持。抗菌活性以及细胞相容性测定的结果证实了酶制剂的结构和功能完整性以及电纺纳米纤维的生物相容性，从而提供了更适合用作伤口清创的敷料。

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