• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >Transcriptional regulation of the claudin-16 gene by Mg ~(2+)availability
【24h】

Transcriptional regulation of the claudin-16 gene by Mg ~(2+)availability

机译：Mg〜（2+）有效性对claudin-16基因的转录调控

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background/Aims: Renal tubular Mg~(2+) reabsorption is mediated predominantly by the tight junction channel protein claudin-16 which is encoded by the gene CLDN16. Hypermagnesemia decreases, whereas hypomagnesemia increases Mg~(2+) reabsorption. This study examines the role of claudin-16 in the adaptive response of the kidney to Mg~(2+) availability. Methods/Results: Mice received a low-, normal-or high Mg~(2+) diet for up to 3 days. Mg~(2+)-loaded animals displayed hypermagnesemia with increasing urine Mg~(2+)/Ca~(2+) levels paralleled by a decrease in claudin-16 protein and mRNA in the kidney. Mg~(2+)-deprived animals developed hypomagnesemia with decreasing urine Mg~(2+)/Ca~(2+) levels associated with an increase in claudin-16 protein and mRNA abundance. Mg~(2+) depletion markedly increased and Mg~(2+) load decreased endogenous claudin-16 mRNA levels in calcium-sensing receptor-transfected HEK293 cells compared with native HEK293 cells. The effect of Mg~(2+) availability on the human CLDN16 (hCLDN16) gene promoter was examined. Using a 2.5kb hCLDN16 5′-flanking DNA sequence, we show that magnesium depletion increases and Mg~(2+) load decreases hCLDN16 promoter activity in transfected HEK293 cells. Conclusions: Changes in Mg~(2+) availability may influence claudin-16 mediated Mg~(2+) transport at the transcriptional level. The possible involvement of the cell membrane bound Ca~(2+)/Mg~(2+) sensing receptor or the potential role of a hypothetical Mg~(2+) response element on the CLDN16 promoter in the Mg~(2+)-induced response remains to be explored.
机译：背景/目的：肾小管Mg〜（2+）的重吸收主要是由CLDN16基因编码的紧密连接通道蛋白claudin-16介导的。高镁血症减少，而低镁血症增加Mg〜（2+）的重吸收。本研究探讨了claudin-16在肾脏对Mg〜（2+）利用率的适应性反应中的作用。方法/结果：小鼠接受低，正常或高Mg〜（2+）饮食长达3天。装载有Mg〜（2+）的动物表现出高镁血症，尿Mg〜（2 +）/ Ca〜（2+）水平升高，同时肾脏中claudin-16蛋白和mRNA降低。缺乏Mg〜（2+）的动物发生低镁血症，尿Mg〜（2 +）/ Ca〜（2+）含量降低，这与claudin-16蛋白和mRNA丰度增加有关。与天然HEK293细胞相比，钙敏感受体转染的HEK293细胞中Mg〜（2+）的消耗显着增加，Mg〜（2+）的负载降低内源性claudin-16 mRNA水平。研究了Mg〜（2+）可用性对人CLDN16（hCLDN16）基因启动子的影响。使用2.5kb hCLDN16 5'侧翼DNA序列，我们表明在转染的HEK293细胞中，镁耗竭增加而Mg〜（2+）负载降低了hCLDN16启动子活性。结论：Mg〜（2+）可用性的变化可能在转录水平上影响claudin-16介导的Mg〜（2+）转运。细胞膜结合的Ca〜（2 +）/ Mg〜（2+）感应受体的可能参与或假设的Mg〜（2+）反应元件在Mg〜（2+）中CLDN16启动子上的潜在作用诱导的反应仍有待探索。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号