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首页> 外文期刊>RSC Advances >Pectin coated polyaniline nanoparticles for an amperometric glucose biosensor
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Pectin coated polyaniline nanoparticles for an amperometric glucose biosensor

机译：果胶涂层的聚苯胺纳米颗粒用于安培型葡萄糖生物传感器

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摘要

An amperometric glucose sensor based on a nanoparticulate form of polyaniline (PAni) is reported herein. The PAni nanoparticles were synthesized using pectin (PAni-Pec NPs) and functionalized with glucose oxidase (GOx-PAni-Pec NPs) to create a highly specific biosensor. Biopolymer pectin stabilizes the colloidal nanoparticles of PAni while also allowing homogenous and a high degree of functionalization of the nanoparticles with glucose oxidase via covalent coupling. This helped to arrest enzyme leaching and enhance biosensor stability. The biosensor showed high sensitivity (79.49 mu A mM(-1) cm(-2)), wide linear range (0.06-4 mM) and a low detection limit (43.5 mu M) at a working potential of 0.6 V. The sensitivity exhibited by GOx-PAni-Pec NPs reported herein was three times higher than that of conventional polyaniline for the same amount of GOx immobilized by a physical adsorption method. Efficient loading and organization of GOx on the nanoparticles could afford adequate availability of the substrate to the enzyme sites leading to better sensitivity. The biosensor did not respond to the presence of electroactive interferents (ascorbic acid, urea, and uric acid) coexisting with glucose in physiological fluids, thereby revealing its specificity. The analytical performance of the biosensor was evaluated for estimation of glucose in blood serum samples. The reliability and stability of the glucose biosensor indicate its potential for application in routine analysis of glucose in physiological fluids.
机译：本文报道了基于聚苯胺（PAni）的纳米颗粒形式的安培葡萄糖传感器。使用果胶（PAni-Pec NPs）合成PAni纳米颗粒，并用葡萄糖氧化酶（GOx-PAni-Pec NPs）功能化，以创建高度特异性的生物传感器。生物聚合物果胶稳定了PAni的胶体纳米颗粒，同时还允许通过共价偶联用葡萄糖氧化酶对纳米颗粒进行均质且高度官能化。这有助于阻止酶浸出并增强生物传感器的稳定性。该生物传感器在0.6 V的工作电压下显示出高灵敏度（79.49μA mM（-1）cm（-2）），宽线性范围（0.06-4 mM）和低检测限（43.5μM）。对于通过物理吸附方法固定的相同量的GOx，本文报道的GOx-PAni-Pec NPs所显示的NP活性是常规聚苯胺的三倍。 GOx在纳米颗粒上的有效负载和组织可以为酶位点提供足够的底物利用率，从而提高灵敏度。该生物传感器对生理流体中与葡萄糖共存的电活性干扰物（抗坏血酸，尿素和尿酸）的存在没有反应，从而揭示了其特异性。评价生物传感器的分析性能以评估血清样品中的葡萄糖。葡萄糖生物传感器的可靠性和稳定性表明其在常规分析生理液中葡萄糖方面的潜力。

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