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首页> 外文期刊>RSC Advances >Precisely designed rattle-type mTiO(2)@P(NIPAM-co-MBA) microspheres with screening gel network for highly selective extraction of phosphopeptidome
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Precisely designed rattle-type mTiO(2)@P(NIPAM-co-MBA) microspheres with screening gel network for highly selective extraction of phosphopeptidome

机译：精确设计的拨浪鼓型mTiO（2）@P（NIPAM-co-MBA）微球，具有筛选凝胶网络，可高度选择性地提取磷酸肽组

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The selective extraction of phosphopeptidome from complicated biological samples is of great importance for the development of diagnostic and prognostic biomarkers, but still remains a challenge. In this work, rattle-type mTiO(2)@P(NIPAM-co-MBA) composite microspheres comprising a mesoporous crystalline mTiO(2) core, an intermediate hollow space and a crosslinked P(NIPAM-co-MBA) network shell were elaborately designed and fabricated via two-step reflux-precipitation polymerization followed by a hydrothermal process. First, a non-crosslinked PMAA layer was directly coated onto the surface of the TiO2 core without any pretreatment. Then, the formed TiO2@PMAA was encapsulated with another crosslinked P(NIPAM-co-MBA) layer with the aid of the strong hydrogen-bonding interaction between the two polymer layers. Finally, a hydrothermal process was adopted to convert the TiO2 core into a crystalline and mesoporous counterpart. At the same time, a non-crosslinked PMAA layer was selectively removed to form a rattle-type structure. The crosslinked P(NIPAM-co-MBA) shell makes the rattle-type mTiO(2)@P(NIPAM-co-MBA) possess great size-exclusion effect against both high-molecular-weight nonphosphoproteins and high-molecular-weight phosphoproteins ,while the mTiO(2) core was responsible of the selective enrichment of the low-molecular-weight phosphopeptides. With the help of these unique properties, the rattle-type mTiO(2)@P(NIPAM-co-MBA) microspheres show excellent potential for the one-step selective extraction of the phosphopeptidome.
机译：从复杂的生物样品中选择性提取磷酸肽组对诊断和预后生物标志物的开发非常重要，但仍然是一个挑战。在这项工作中，拨浪鼓型mTiO（2）@P（NIPAM-co-MBA）复合微球包括介孔晶体mTiO（2）核，中间的中空空间和交联的P（NIPAM-co-MBA）网络壳通过两步回流沉淀聚合和水热工艺精心设计和制造。首先，将未经交联的PMAA层直接涂覆在TiO2核的表面上，而无需任何预处理。然后，借助两个聚合物层之间的强氢键相互作用，将形成的TiO2 @ PMAA封装在另一个交联的P（NIPAM-co-MBA）层中。最后，采用水热法将TiO2核转化为结晶和介孔的对应物。同时，非交联的PMAA层被选择性地去除以形成拨浪鼓型结构。交联的P（NIPAM-co-MBA）壳使拨浪鼓型mTiO（2）@P（NIPAM-co-MBA）对高分子量非磷酸蛋白和高分子量磷酸蛋白均具有很大的尺寸排阻作用，而mTiO（2）核心负责低分子量磷酸肽的选择性富集。借助这些独特的特性，拨浪鼓型mTiO（2）@P（NIPAM-co-MBA）微球显示了一步选择性提取磷酸肽组的巨大潜力。

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