• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >A fluorescent biosensor of lysozyme-stabilized copper nanoclusters for the selective detection of glucose
【24h】

A fluorescent biosensor of lysozyme-stabilized copper nanoclusters for the selective detection of glucose

机译：溶菌酶稳定的铜纳米簇的荧光生物传感器，用于葡萄糖的选择性检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glucose biosensors have attracted increased attention, as the rapid and sensitive detection of glucose is highly desirable for diabetes diagnosis. In this article, we designed a type of lysozyme functionalized fluorescence copper nanoclusters (Lys-CuNCs) to detect glucose levels in blood samples. Fluorescence measurements were carried out to optimize the synthesis conditions (e.g. mass ratio, pH and reaction time) for the biosensor. Under optimum conditions, the obtained Lys-CuNCs with an average diameter of 2 nm exhibited bright orangey-red fluorescence with high quantum yields (up to 5.6%). The fluorescence signal of Lys-CuNCs was quenched upon the addition of glucose, presumably due to the reduction of Cu(I) on the NCs surface by glucose. Thus the Lys-CuNCs can be served as a biosensor for glucose detection and two linear response ranges respectively in 0.03-10 mu M and 0.5-10 mM of glucose were observed with a detection limit of 1.9 nM. Furthermore, this biosensor showed superior selectivity for various interferences, including light radiation, metal ions, carbohydrates and amino acids. In view of these properties, the Lys-CuNCs biosensor was applied in the determination of glucose in blood samples, and the results agreed well with that obtained from a currently used clinical method. Finally the visualized fluorescence variation of Lys-CuNCs may further enable the rapid and simple detection of glucose level in blood.
机译：葡萄糖生物传感器已引起越来越多的关注，因为快速，灵敏地检测葡萄糖对于糖尿病的诊断是非常需要的。在本文中，我们设计了一种溶菌酶功能化的荧光铜纳米簇（Lys-CuNCs），用于检测血液样本中的葡萄糖水平。进行荧光测量以优化生物传感器的合成条件（例如质量比，pH和反应时间）。在最佳条件下，获得的平均直径为2 nm的Lys-CuNCs表现出明亮的橙红色荧光，具有很高的量子产率（高达5.6％）。添加葡萄糖后，Lys-CuNCs的荧光信号被淬灭，这可能是由于葡萄糖在NCs表面还原了Cu（I）所致。因此，Lys-CuNCs可以用作葡萄糖检测的生物传感器，观察到分别在0.03-10μM和0.5-10 mM的葡萄糖中有两个线性响应范围，检测限为1.9 nM。此外，该生物传感器对各种干扰（包括光辐射，金属离子，碳水化合物和氨基酸）表现出优异的选择性。考虑到这些特性，Lys-CuNCs生物传感器被用于测定血液样本中的葡萄糖，其结果与从目前使用的临床方法获得的结果非常吻合。最后，Lys-CuNCs的可视化荧光变化可以进一步实现对血液中葡萄糖水平的快速简单检测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号