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A new collagen solution with high concentration and collagen native structure perfectly preserved

机译：完美保存高浓度和胶原天然结构的新型胶原溶液

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摘要

Collagen, the most abundant protein in mammals, is widely used for making biomaterials. Usually, organic solvents, such as 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), or acids (H3PO4, HAc) have been used to disperse collagen to make collagen-based biomaterials. However, the native structure of collagen is often seriously damaged and the concentration of collagen in solution was always relatively low, which greatly limited its application. In this work, we have firstly made a detailed study of the effect of H+ and HAc on collagen dispersal in terms of Zeta potential, particle size and Circular Dichroism (CD), followed by using a benign sodium acetate/acetic acid buffer solution, which is proposed to disperse the collagen. The results showed that the collagen solution from a NaAc/HAc buffer solution at pH = 3.0 had both high concentration (100 mg mL(-1)) and perfect native structure preservation (up to 94%). We demonstrated that it was the constant concentration of free H+ in the NaAc/HAc buffer solution whose pH was fixed at 3.0 that can maintain an unchanged amount of surface charge on the collagen colloidal particles, which enables the collagen to be dispersed well even at high dose. As an application, the collagen solution from the NaAc/HAc solution at pH 3.0 was successfully electrospun into nanofibers, and the obtained collagen nanofibers can still maintain as much as 57% of its native structure. This study indicated that the novel buffer solution of NaAc/HAc at pH 3.0 could be commonly used in the processing of collagen for a variety of biomaterials based on collagen solutions, and has great potential for use in tissue engineering.
机译：胶原蛋白是哺乳动物中最丰富的蛋白质，被广泛用于制造生物材料。通常，已经使用有机溶剂，例如1,1,1,3,3,3-六氟-2-丙醇（HFIP）或酸（H3PO4，HAc）来分散胶原蛋白，从而制成基于胶原蛋白的生物材料。但是，胶原蛋白的天然结构经常受到严重破坏，溶液中的胶原蛋白浓度始终相对较低，极大地限制了其应用。在这项工作中，我们首先从Zeta电位，粒径和圆二色性（CD）方面详细研究了H +和HAc对胶原分散的影响，然后使用良性乙酸钠/乙酸缓冲溶液，建议分散胶原蛋白。结果表明，pH = 3.0的NaAc / HAc缓冲溶液中的胶原蛋白溶液既具有高浓度（100 mg mL（-1）），又具有完美的天然结构保存（高达94％）。我们证明了NaAc / HAc缓冲溶液中的游离H +浓度恒定，其pH值固定为3.0，可以保持胶原胶体颗粒上的表面电荷量不变，即使在高温下也能很好地分散胶原蛋白剂量。作为应用，将来自pH 3.0的NaAc / HAc溶液的胶原蛋白溶液成功地电纺成纳米纤维，并且所得的胶原蛋白纳米纤维仍可以保持其天然结构的57％。这项研究表明，pH 3.0的新型NaAc / HAc缓冲溶液可以普遍用于处理多种基于胶原蛋白溶液的生物材料的胶原蛋白，并且在组织工程中具有巨大的潜力。

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