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首页> 外文期刊>RSC Advances >A label-free electrochemical immunosensor for carcinoembryonic antigen detection on a graphene platform doped with poly(3,4-ethylenedioxythiophene)/Au nanoparticles
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A label-free electrochemical immunosensor for carcinoembryonic antigen detection on a graphene platform doped with poly(3,4-ethylenedioxythiophene)/Au nanoparticles

机译：一种无标记的电化学免疫传感器，用于在掺杂有聚（3,4-乙撑二氧噻吩）/ Au纳米粒子的石墨烯平台上检测癌胚抗原

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摘要

In this work, a two-step method was developed for the fabrication of a graphene sensing platform doped with poly(3,4-ethylenedioxythiophene)/Au nanoparticles (AuNPs/PEDOT/GR). PEDOT nanorods grown on graphene oxide nanosheets (PEDOT/GO) were firstly synthesized by liquid-liquid interfacial polymerization, followed by the chemical reduction of HAuCl4 by NaBH4. During the reduction process, GO doped in the PEDOT was also reduced to a more conductive form of GR. The obtained AuNPs/PEDOT/GR showed excellent conductivity and large surface area. Thus, a simple and sensitive label-free immunosensor based on AuNPs/PEDOT/GR nanocomposite has been proposed to detect carcinoembryonic antigen (CEA) by measuring the change of electrochemical response before and after the immunoreaction. Under the optimized conditions, the linear range of the proposed immunosensor is estimated to be from 0.0004 to 40 ng mL(-1) (R-2 = 0.9969) and the detection limit is estimated to be 0.1 pg mL(-1) at a signal-to-noise ratio of 3. Moreover, the immunosensor was examined for use in the determination of CEA in real human serum samples with satisfactory results.
机译：在这项工作中，开发了一种分两步的方法来制造掺杂有聚（3,4-乙撑二氧噻吩）/ Au纳米粒子（AuNPs / PEDOT / GR）的石墨烯传感平台。首先通过液-液界面聚合反应，然后用NaBH4对HAuCl4进行化学还原，合成了在氧化石墨烯纳米片（PEDOT / GO）上生长的PEDOT纳米棒。在还原过程中，掺杂在PEDOT中的GO也被还原为导电性更高的GR形式。所得的AuNPs / PEDOT / GR显示出优异的导电性和大的表面积。因此，已经提出了一种基于AuNPs / PEDOT / GR纳米复合材料的简单且灵敏的无标记免疫传感器，通过测量免疫反应前后的电化学反应变化来检测癌胚抗原（CEA）。在优化的条件下，建议的免疫传感器的线性范围估计为0.0004至40 ng mL（-1）（R-2 = 0.9969），检测限估计为0.1 pg mL（-1）。信噪比为3。此外，检查了该免疫传感器用于测定真实人血清样品中CEA的效果，结果令人满意。

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