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首页> 外文期刊>RSC Advances >Colorimetric sensing of sinapine based on competitive interaction of papain-coated gold nanoparticles and sinapine toward Pb2+
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Colorimetric sensing of sinapine based on competitive interaction of papain-coated gold nanoparticles and sinapine toward Pb2+

机译：基于涂有木瓜蛋白酶的金纳米颗粒和sinapine对Pb2 +的竞争相互作用对sinapine进行比色传感

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摘要

Direct selective and sensitive sensing of sinapine (SA) in diets and plants is of great importance due to its crucial roles in the functions of life. In this study, we demonstrate a sensitive and selective colorimetric method for SA sensing in rapeseed with papain-coated gold nanoparticles (P-AuNPs) as signal readouts based on the competitive interaction of papain and SA toward Pb2+. Initially, P-AuNPs are wine red in color and exhibit absorption at 524 nm (A(524)) in the UV-vis spectra. The addition of an aqueous solution of Pb2+ to the P-AuNPs dispersion triggers the aggregation of P-AuNPs, resulting in the wine-red-to-blue color change and the appearance of a new absorption peak at 626 nm (A(626)) in the UV-vis absorption spectra. The addition of SA into P-AuNPs and Pb2+ (AuNPs/Pb2+) solution induces changes in both the color and the UV-vis spectra of the P-AuNPs aggregation because of the strong binding between Pb2+ and SA. Thus, the colorimetric sensing of SA can be realized through the blue-to-wine-red color change of the P-AuNPs solution and measuring the ratio of absorbance at a wavelength of 524 nm to that at 626 nm in the UV-vis spectra. The study demonstrated here offers a simple yet effective method for SA assay and expands the applications of AuNPs in bioassays.
机译：在饮食和植物中对西那平（SA）进行直接选择性和敏感的传感非常重要，因为它在生命功能中起着至关重要的作用。在这项研究中，我们基于木瓜蛋白酶和SA对Pb2 +的竞争性相互作用，展示了一种敏感且选择性的比色法，用于油菜籽中涂有木瓜蛋白酶的金纳米颗粒（P-AuNPs）作为信号读出的SA传感。最初，P-AuNPs为酒红色，在紫外可见光谱中显示在524 nm（A（524））处有吸收。在P-AuNPs分散液中添加Pb2 +水溶液会触发P-AuNPs的聚集，导致酒红色到蓝色的变化，并在626 nm处出现新的吸收峰（A（626） ）在紫外可见吸收光谱中。在P-AuNPs和Pb2 +（AuNPs / Pb2 +）溶液中添加SA会导致P-AuNPs聚集体的颜色和UV-vis光谱发生变化，因为Pb2 +和SA之间具有很强的结合力。因此，SA的比色感测可以通过P-AuNPs溶液的蓝到酒红色变色并测量紫外可见光谱中524 nm波长与626 nm波长的吸光度之比来实现。此处展示的研究为SA分析提供了一种简单而有效的方法，并扩展了AuNPs在生物分析中的应用。

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