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首页> 外文期刊>RSC Advances >A non-covalent complex of quantum dots and chlorin e(6): efficient energy transfer and remarkable stability in living cells revealed by FLIM
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A non-covalent complex of quantum dots and chlorin e(6): efficient energy transfer and remarkable stability in living cells revealed by FLIM

机译：量子点和二氢卟酚e（6）的非共价复合物：有效的能量转移和FLIM显示的活细胞显着稳定性

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A Forster resonance energy transfer (FRET) system of semiconductor quantum dots and porphyrins represents a new promising photosensitizing tool for the photodynamic therapy of cancer. In this work, we demonstrate the ability of a non-covalent complex formed between commercial lipid-coated CdSe/ ZnS quantum dots (QD) bearing different terminal groups (carboxyl, amine or non-functionalized) and a second-generation photosensitizer, chlorin e(6) (Ce-6) to enter living HeLa cells with maintained integrity and perform FRET from two-photon excited QD to bound Ce-6 molecules. Spectroscopic changes, the highly efficient FRET, observed upon Ce-6 binding to QD, and remarkable stability of the QD-Ce-6 complex in different media suggest that Ce-6 penetrates inside the lipid coating close to the inorganic core of QD. Two-photon fluorescence lifetime imaging microscopy (FLIM) on living HeLa cells revealed that QD-Ce-6 complexes localize within the plasma membrane and intracellular compartments and preserve high FRET efficiency (similar to 50%). The latter was confirmed by recovery of QD emission lifetime after photobleaching of Ce-6. The intracellular distribution pattern and FRET efficiency of QD-Ce-6 complexes did not depend on the charge of QD terminal groups. Given the non-covalent nature of the complex, its exceptional stability in cellulo can be explained by a combination of hydrophobic interactions and coordination of carboxyl groups of Ce6 with the ZnS shell of QD. These findings suggest a simple route to the preparation of QD-photosensitizer complexes featuring efficient FRET and high stability in cellulo without using time-consuming conjugation protocols.
机译：半导体量子点和卟啉的Forster共振能量转移（FRET）系统代表了一种新的有前途的光敏工具，用于癌症的光动力治疗。在这项工作中，我们证明了带有不同末端基团（羧基，胺或未官能化）的商业脂质包覆的CdSe / ZnS量子点（QD）与第二代光敏剂二氢卟酚之间形成非共价复合物的能力。 （6）（Ce-6）进入保持完整性的活HeLa细胞，并从双光子激发的QD到结合的Ce-6分子进行FRET。 Ce-6与QD结合后观察到的光谱变化，高效FRET，以及QD-Ce-6复合物在不同介质中的显着稳定性表明，Ce-6渗透到脂质包裹的QD无机核附近。对活HeLa细胞的双光子荧光寿命成像显微镜（FLIM）显示，QD-Ce-6复合物位于质膜和细胞内区室内，并保持较高的FRET效率（约50％）。后者通过Ce-6光漂白后QD发射寿命的恢复得到证实。 QD-Ce-6复合物的胞内分布模式和FRET效率不取决于QD末端基团的电荷。鉴于复合物的非共价性质，其在纤维素中的出色稳定性可以通过疏水相互作用和Ce6的羧基与QD的ZnS壳的配位相结合来解释。这些发现提示了制备QD-光敏剂复合物的简单方法，该复合物具有高效的FRET和纤维素的高稳定性，而无需使用费时的偶联方案。

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