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首页> 外文期刊>Biological chemistry >The two SH2-domain-containing inositol 5-phosphatases SHIP1 and SHIP2 are coexpressed in human T lymphocytes.
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The two SH2-domain-containing inositol 5-phosphatases SHIP1 and SHIP2 are coexpressed in human T lymphocytes.

机译：两种含有SH2结构域的肌醇5-磷酸酶SHIP1和SHIP2在人类T淋巴细胞中共表达。

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摘要

The activation of many hematopoietic cells via cytokine receptors, as well as B and T cell receptors, leads to the tyrosine phosphorylation of Shc and its association with both Grb2-Sos1 complexes and with a 145 kDa protein referred to as the SH2 containing inositol 5-phosphatase (SHIP1). In a search of putative 5-phosphatase isoenzymes, we have isolated a second SH2 domain containing inositol 5-phosphatase, referred to as (SHIP2). Both SHIP1 and SHIP2 are coexpressed in human T lymphocytes. This was shown at the protein level by Western blot analysis in transformed T cell lines and in peripheral blood T lymphocytes either unstimulated or after in vitro activation through TCR-CD3 complex. SHIP1 protein level was not modulated after activation of T lymphocytes, in contrast to SHIP2, which was increased after long-term stimulation. SHIP1 was tyrosine phosphorylated in resting naive T cells. This was not observed in the transformed T cell lines. T lymphocyte is therefore a model of coexpression of the two SH2-containing inositol 5-phosphatases SHIP1 and SHIP2.
机译：许多造血细胞通过细胞因子受体以及B细胞和T细胞受体的活化导致Shc的酪氨酸磷酸化，并与Grb2-Sos1复合物和145 kDa蛋白（称为SH2含肌醇5）结合磷酸酶（SHIP1）。在推定的5-磷酸酶同工酶的搜索中，我们分离了另一个包含肌醇5-磷酸酶的SH2结构域，称为（SHIP2）。 SHIP1和SHIP2在人类T淋巴细胞中共表达。通过蛋白质印迹分析在未刺激的T细胞系和通过TCR-CD3复合物体外活化后的转化T细胞系和外周血T淋巴细胞的蛋白质水平上显示了这一点。 T细胞活化后，SHIP1蛋白水平未受到调节，而SHIP2则在长期刺激后增加。 SHIP1在静止的幼稚T细胞中被酪氨酸磷酸化。这在转化的T细胞系中未观察到。因此，T淋巴细胞是两种含SH2的肌醇5磷酸酶SHIP1和SHIP2共表达的模型。

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