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HPLC-ICP-MS analysis of selenium speciation in selenium-enriched Cordyceps militaris

机译：富硒冬虫夏草中硒形态的HPLC-ICP-MS分析

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This study examined the distribution of selenium in proteins from selenium-enriched Cordyceps militaris extract using reversed-phase high performance liquid chromatography (RP-HPLC) and size-exclusion chromatography (SEC) coupled to on-line ICP-MS specific for selenium detection. An analytical method was developed for detecting the presence of selenocysteine, selenomethionine and selenopeptides. The results showed that soluble selenium was the major form of selenium in selenium-enriched C. militaris extract. About 84.4% of the selenium was present as low-molecular-weight molecules (MW < 4 kDa), whereas 7.13% of the selenium was present in the form of high-molecular-weight molecules, probably as selenoproteins. Furthermore, analysis of acid-hydrolyzed C. militaris extract revealed a higher proportion of Se-Cys2 than Se-Met (two main selenium-conjugated amino acids) in the extract, although various forms of smaller selenium-proteins or selenium-peptides were also present in low but detectable amounts. The present analysis of selenium thus combined different chromatographic techniques for efficient and rapid separation of selenoproteins and other seleno-conjugated molecules.
机译：这项研究使用反相高效液相色谱（RP-HPLC）和体积排阻色谱（SEC）结合专门用于硒检测的在线ICP-MS，研究了富硒Cord虫草提取物中蛋白质中硒的分布。开发了一种分析方法来检测硒代半胱氨酸，硒代蛋氨酸和硒肽的存在。结果表明，可溶性硒是富硒C. militaris提取物中硒的主要形式。硒的约84.4％以低分子量分子（MW <4 kDa）的形式存在，而硒的7.13％以高分子量分子的形式存在，可能以硒蛋白的形式存在。此外，对酸水解的C.militaris提取物进行分析后发现，提取物中Se-Cys2的比例高于Se-Met（两个主要的硒共轭氨基酸），尽管各种形式的较小硒蛋白或硒肽也存在。含量低但可检测。因此，目前对硒的分析结合了不同的色谱技术，可以有效，快速地分离硒蛋白和其他硒共轭分子。

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