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首页> 外文期刊>RSC Advances >Surface-enhanced Raman spectroscopic detection and differentiation of lung cancer cell lines (A549, H1229) and normal cell line (AT II) based on gold nanostar substrates
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Surface-enhanced Raman spectroscopic detection and differentiation of lung cancer cell lines (A549, H1229) and normal cell line (AT II) based on gold nanostar substrates

机译：基于金纳米星底物的肺癌细胞系（A549，H1229）和正常细胞系（AT II）的表面增强拉曼光谱检测和分化

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摘要

In this paper, a non-invasive, simple and efficient approach is constructed to make clean gold nanostar substrates with higher enhancement effect, homogeneous surface-enhanced Raman scattering (SERS) activity and high reproducibility, which are the assembly of gold nanostars based on the electrostatically assisted APTES-functionalized surface-assembly method. The obtained substrates enable us to successfully compare and distinguish two lung cancer cell lines [human lung adenocarcinoma epithelial cell line (A549), human non-small cell lung cancer cell line (H1229)] and one normal lung cell line [human type II alveolar epithelial cell line (AT II)] through SERS spectra. The difference spectra (A549 - H1229, A549 - AT II and H1229 - AT II) among the three cell lines are studied to better interpret the spectral differences and gain insight into the biochemical variation. Furthermore, principal component analysis (PCA) score plots of the SERS spectra are also used to better view the differences among these three cell lines. This sensitive, efficient and non-invasive detection method, based on gold nanostar substrates, will have great potential for the differentiation of living cells and play an important role in the early diagnosis and treatment of cancers.
机译：本文构建了一种非侵入性，简单而有效的方法来制造具有更高增强效果，均一的表面增强拉曼散射（SERS）活性和高重现性的清洁金纳米星基底，这些基底是基于纳米金星的组装。静电辅助APTES功能化表面组装方法。获得的底物使我们能够成功比较和区分两种肺癌细胞系[人肺腺癌上皮细胞系（A549），人非小细胞肺癌细胞系（H1229）]和一种正常肺细胞系[人II型肺泡]上皮细胞系（AT II）]。研究了三种细胞系之间的差异光谱（A549-H1229，A549-AT II和H1229-AT II），以更好地解释光谱差异并深入了解生化变化。此外，SERS光谱的主成分分析（PCA）评分图还用于更好地查看这三种细胞系之间的差异。这种基于金纳米星底物的灵敏，有效且无创的检测方法，对于活细胞的分化将具有巨大的潜力，并在癌症的早期诊断和治疗中发挥重要作用。

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