Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

Zholobak Nadezhda M.; Shcherbakov Alexander B.; Vitukova Ekaterina O.; Yegorova Alla V.; Scripinets Yulia V.; Leonenko Inna I.; Baranchikov Alexander Ye.; Antonovich Valeriy P.; Ivanov Vladimir K.

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Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

机译：直接监测活性细胞中ROS与二氧化铈纳米颗粒之间的相互作用

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A method for the direct monitoring of the interaction of reactive oxygen species (ROS) with cerium dioxide nanoparticles (CDN) in living cells is proposed based on the use of a complex of calcein and CDN. The CDN-calcein complex penetrates easily into a living cell and is decomposed readily by endogenous or exogenous ROS, releasing brightly fluorescent calcein, which can be observed using conventional fluorescent microscopy. This complex is less cytotoxic than individual cerium dioxide nanoparticles and provides effective protection from oxidative stress induced by the action of hydrogen peroxide, treatment with latex beads, or infection by the vesicular stomatitis virus.

机译：基于钙黄绿素和CDN的复合物，提出了一种直接监测活细胞中活性氧（ROS）与二氧化铈纳米颗粒（CDN）相互作用的方法。 CDN-钙黄绿素复合物容易渗透到活细胞中，并容易被内源性或外源性ROS分解，释放出明亮的荧光钙黄绿素，可以使用常规荧光显微镜观察到。该复合物比单个二氧化铈纳米粒子的细胞毒性要小，并且可以有效保护免受过氧化氢，乳胶珠治疗或水疱性口炎病毒感染引起的氧化应激。

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《RSC Advances》 |2014年第93期|共8页
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Zholobak Nadezhda M.; Shcherbakov Alexander B.; Vitukova Ekaterina O.; Yegorova Alla V.; Scripinets Yulia V.; Leonenko Inna I.; Baranchikov Alexander Ye.; Antonovich Valeriy P.; Ivanov Vladimir K.;
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1. Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells [J] . Zholobak Nadezhda M., Shcherbakov Alexander B., Vitukova Ekaterina O., RSC Advances . 2014,第93期

机译：直接监测活性细胞中ROS与二氧化铈纳米颗粒之间的相互作用
2. Electrostatic interactions for directed assembly of nanostructured materials: composites of titanium dioxide nanotubes with gold nanoparticles [J] . Jingxian Yu, Graham A. Rance, Andrei N. Khlobystov Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology . 2009,第47期

机译：纳米结构材料的定向组装的静电相互作用：二氧化钛纳米管与金纳米颗粒的复合物
3. Cerium dioxide (CeO_2) nanoparticles decrease arsenite (As(Ⅲ)) cytotoxicity to 16HBE14o- human bronchial epithelial cells [J] . Zeng Chao, Chi Nguyen, Boitano Scott, Environmental research . 2018,第JULa期

机译：二氧化铈（CeO_2）纳米颗粒降低亚砷酸盐（As（Ⅲ））对16HBE14o-人支气管上皮细胞的细胞毒性
4. Chapter 23 Cerium Dioxide CeO_(2-x) and Orthovanadate (Gd_(0.9)Eu_(0.1)VO_4) Nanoparticles for Protection of Living Body from X-Ray Induced Damage [C] . G. Grygorova, V. Klochkov, Ye. Mamotyuk, NATO Advanced Research Workshop on Nanomaterials for Security . 2016

机译：第23章二氧化铈CEO_（2-X）和脱芳酸盐（GD_（0.9）EU_（0.1）VO_4）纳米粒子，用于保护生物体免受X射线诱导的损伤
5. Functionalized microfluidic devices for directing and monitoring cell-material interactions. [D] . Simms, Helen Marie. 2009

机译：功能化的微流控设备，用于指导和监测细胞与材料的相互作用。
6. Combinatory Effects of Cerium Dioxide Nanoparticles and Acetaminophen on the Liver—A Case Study of Low-Dose Interactions in Human HuH-7 Cells [O] . Benjamin C. Krause, Fabian L. Kriegel, Victoria Tartz, 2021

机译：二氧化铈纳米粒子和对乙酰氨基酚对肝脏的组合作用 - 一种人HUH-7细胞低剂量相互作用的案例研究
7. Combinatory Effects of Cerium Dioxide Nanoparticles and Acetaminophen on the Liver—A Case Study of Low-Dose Interactions in Human HuH-7 Cells [O] . Benjamin C. Krause, Fabian L. Kriegel, Victoria Tartz, 2021

机译：二氧化铈纳米粒子和对乙酰氨基酚对肝脏的组合作用 - 一种人HUH-7细胞低剂量相互作用的案例研究

Direct monitoring of the interaction between ROS and cerium dioxide nanoparticles in living cells

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