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首页> 外文期刊>Biological chemistry >Characterization of plant miRNAs and small RNAs derived from potato spindle tuber viroid (PSTVd) in infected tomato.
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Characterization of plant miRNAs and small RNAs derived from potato spindle tuber viroid (PSTVd) in infected tomato.

机译:表征受感染番茄中马铃薯纺锤块茎类病毒(PSTVd)的植物miRNA和小RNA。

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摘要

To defend against invading pathogens, plants possess RNA silencing mechanisms involving small RNAs (miRNAs, siRNAs). Also viroids - plant infectious, non-coding, unencapsidated RNA - cause the production of viroid-specific small RNAs (vsRNA), but viroids do escape the cytoplasmic silencing mechanism. Viroids with minor sequence variations can produce different symptoms in infected plants, suggesting an involvement of vsRNAs in symptom production. We analyzed by deep sequencing the spectrum of vsRNAs induced by the PSTVd strain AS1, which causes strong symptoms such as dwarfing and necrosis upon infection of tomato plants cv Rutgers. Indeed, vsRNAs found with highest frequency mapped to the pathogenicity-modulating domain of PSTVd, supporting an involvement of vsRNAs in symptom production. Furthermore, in PSTVd AS1-infected plants the accumulation of some endogenous miRNAs, which are involved in leaf development via regulation of transcription factors, is suppressed. The latter finding supports the hypothesis that a miRNA-dependent (mis)regulation of transcription factors causes the viroid symptoms.
机译:为了防御入侵的病原体,植物具有涉及小RNA(miRNA,siRNA)的RNA沉默机制。同样,类病毒-植物传染性的,非编码的,无衣壳的RNA-会引起类病毒特异性小RNA(vsRNA)的产生,但是类病毒确实逃脱了细胞质沉默机制。具有微小序列变异的类病毒可以在受感染植物中产生不同的症状,表明vsRNA参与了症状产生。我们通过深度测序分析了PSTVd菌株AS1诱导的vsRNA的光谱,该菌株在感染番茄植株Rutgers时引起强烈的症状,例如矮化和坏死。实际上,发现的最高频率的vsRNA映射到PSTVd的致病性调节域,支持了vsRNA参与症状产生。此外,在PSTVd AS1感染的植物中,一些内源性miRNA的积累受到抑制,这些内源性miRNA通过调节转录因子参与叶片发育。后一个发现支持以下假设:转录因子的miRNA依赖性(错误)调节会引起类病毒症状。

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