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首页> 外文期刊>Russian journal of genetics >Functional interactions between promoters of neighboring yellow and CG3777 genes in Drosophila melanogaster
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Functional interactions between promoters of neighboring yellow and CG3777 genes in Drosophila melanogaster

机译：果蝇中邻近的黄色和CG3777基因启动子之间的功能相互作用

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摘要

It was shown by us previously that the transcription of the yellow gene can be affected by the promoter of the neighboring gene CG3777, which has a similar expression profile. In the present work, we continued studying the functional interactions between the promoters of the yellow and CG3777 genes in transgenic Drosophila strains. In this work, we used the failure of the yeast activator GAL4 to stimulate transcription from the promoter of the yellow gene for the case when GAL4-binding sites are localized at the 3′-end of the gene. It has been found that, if the 983-bp CG3777 gene promoter is inserted in transgenic strains in the same orientation with the yellow gene promoter, downstream from the sites of the GAL4 activator, the CG3777 promoter provides a strong stimulation of the yellow gene by the GAL4 activator. When the promoters of the yellow and CG3777 genes are inserted in opposite orientations relative to one another, no stimulation of the yellow gene by GAL4 is observed. Additional results obtained in the work demonstrate that the functional interacton between the CG3777 and yellow promoters depends on their mutual orientation and position relative to the GAL4-binding sites.
机译：先前我们已经表明，黄色基因的转录可能受邻近基因CG3777的启动子影响，该基因的表达谱相似。在目前的工作中，我们继续研究转基因果蝇菌株中黄色和CG3777基因启动子之间的功能相互作用。在这项工作中，我们针对GAL4结合位点位于基因3'端的情况，利用酵母激活剂GAL4未能刺激黄色基因启动子转录。已经发现，如果以与黄色基因启动子相同的方向将983bp的CG3777基因启动子插入到转基因菌株中，则在GAL4活化剂位点的下游，CG3777启动子提供了对黄色基因的强烈刺激。 GAL4激活剂。当黄色和CG3777基因的启动子以彼此相反的方向插入时，未观察到GAL4对黄色基因的刺激。在工作中获得的其他结果表明，CG3777和黄色启动子之间的功能性相互作用子取决于它们的相互取向和相对于GAL4结合位点的位置。

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