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Creation of blast disease-resistant rice varieties with modern DNA-markers

机译:利用现代DNA标记创建抗稻瘟病的水稻新品种

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摘要

Based on modern technologies of molecular DNA-markers, blast disease-resistance genes (Pi-ta, Pi-b, Pi-1, Pi-2, and Pi-33) were introgressed and pyramided into domestic rice varieties to give them longterm disease resistance. For that purpose, this case study uses SSR-markers closely linked to these genes, as well as intragenic markers of genes Pi-ta and Pi-b. Multiplex PCR systems were created for simultaneous identification of two resistance genes in the hybrid progeny for the following combinations: Pi-1 + Pi-2, Pi-ta + Pi-b, Pi-ta + Pi-33.
机译:基于现代分子DNA标记技术,将稻瘟病抗性基因(Pi-ta,Pi-b,Pi-1,Pi-2和Pi-33)渗入并融合到国内水稻品种中,使其长期受病。抵抗性。为此,本案例研究使用与这些基因紧密相关的SSR标记,以及基因Pi-ta和Pi-b的基因内标记。创建了多重PCR系统,用于同时鉴定杂种子代中以下组合的两个抗性基因:Pi-1 + Pi-2,Pi-ta + Pi-b,Pi-ta + Pi-33。

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