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首页> 外文期刊>Occupational and environmental medicine >Study on the relation between occupational fenvalerate exposure and spermatozoa DNA damage of pesticide factory workers.
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Study on the relation between occupational fenvalerate exposure and spermatozoa DNA damage of pesticide factory workers.

机译：农药工厂工人职业性氰戊菊酯暴露与精子DNA损伤的关系研究。

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AIMS: To determine sperm nuclear DNA integrity and to investigate the relation between fenvalerate (FE) exposure and spermatozoa DNA damage. METHODS: Sperm DNA fragmentation was detected by a modified alkaline single cell gel electrophoresis (Comet) assay and a terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) assay. The olive tail moment (OTM) and percentage tail DNA were measured by the Comet assay, and cell positive percentage was measured by the TUNEL assay for DNA damage evaluation. RESULTS: The DNA integrity of spermatozoa of external and internal control groups were both significantly greater than that of the FE exposed group. The median value of tail DNA percentage in the exposure group was 11.30, which was significantly higher than 5.60 in the internal control group and 5.10 in the external control group. The median value of OTM was 3.80 in the exposure group, significantly higher than 1.50 in the internal control group and 2.00 in the external control group. Mean cell positive was 31.2% in the exposure group, significantly higher than 17.4% in the internal control and 19.6% in the external control groups. Cell positive (%) was significantly correlated with tail DNA percentage and with OTM of whole subjects (n = 63). CONCLUSIONS: Results showed that occupational FE exposure is associated with an increase in sperm DNA damage. A combination of the Comet and TUNEL assays would offer more comprehensive information for a better understanding of sperm DNA damage, and the biological significance of sperm DNA damage in sperm function and male infertility.
机译：目的：确定精子核DNA的完整性，并研究氰戊菊酯（FE）暴露与精子DNA损伤之间的关系。方法：采用改良的碱性单细胞凝胶电泳（Comet）法和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法检测精子DNA片段。橄榄尾矩（OTM）和尾巴DNA百分比通过Comet分析测量，而细胞阳性百分比通过TUNEL分析测量，以评估DNA损伤。结果：外部和内部对照组精子的DNA完整性均显着高于暴露于FE的组。暴露组的尾巴DNA百分比中位数为11.30，显着高于内部对照组的5.60和外部对照组的5.10。暴露组的OTM中位数为3.80，显着高于内部对照组的1.50和外部对照组的2.00。暴露组的平均细胞阳性率为31.2％，显着高于内部对照组的17.4％和外部对照组的19.6％。细胞阳性（％）与整个受试者的尾巴DNA百分比和OTM显着相关（n = 63）。结论：结果表明，职业性FE暴露与精子DNA损伤增加有关。彗星和TUNEL分析的结合将提供更全面的信息，以更好地了解精子DNA损伤，以及精子DNA损伤对精子功能和男性不育的生物学意义。

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