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首页> 外文期刊>Occupational and environmental medicine >Genetic damage in operating room personnel exposed to isoflurane and nitrous oxide.
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Genetic damage in operating room personnel exposed to isoflurane and nitrous oxide.

机译：暴露于异氟烷和一氧化二氮的手术室人员的遗传损伤。

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OBJECTIVES: To evaluate genetic damage as the frequency of sister chromatid exchanges and micronuclei in lymphocytes of peripheral blood of operating room personnel exposed to waste anaesthetic gases. METHODS: Occupational exposure was measured with a direct reading instrument. Venous blood samples were drawn from 10 non-smokers working in the operating room and 10 non-smoking controls (matched by age, sex, and smoking habits). Lymphocytes were cultured separately over 72 hours for each assay with standard protocols. At the end of the culture time, the cells were harvested, stained, and coded for blind scoring. The exchanges of DNA material were evaluated by counting the number of sister chromatid exchanges in 30 metaphases per probe or by counting the frequency of micronuclei in 2000 binucleated cells. Also, the mitotic and proliferative indices were measured. RESULTS: The operating room personnel at the hospital were exposed to an 8 hour time weighted average of 12.8 ppm nitrous oxide and 5.3 ppm isoflurane. The mean (SD) frequency of sister chromatid exchanges was significantly higher (10.2 (1.9) v 7.4 (2.4)) in exposed workers than controls (p = 0.036) the proportion of micronuclei (micronuclei/500 binucleated cells) was also higher (8.7 (2.9) v 6.8 (2.5)), but was not significant (p = 0.10). CONCLUSION: Exposure even to trace concentrations of waste anaesthetic gases may cause dose-dependent genetic damage. Concerning the micronuclei test, no clastogenic potential could be detected after average chronic exposure to waste anaesthetic gas. However, an increased frequency of sister chromatid exchanges in human lymphocytes could be detected. Although the measured differences were low, they were comparable with smoking 11-20 cigarettes a day. Due to these findings, the increased proportion of micronuclei and rates of sister chromatid exchanges may be relevant long term and need further investigation.
机译：目的：以暴露于麻醉性麻醉气体的手术室人员外周血淋巴细胞的姐妹染色单体交换和微核的频率评估遗传损伤。方法：使用直读仪测量职业暴露量。从手术室工作的10名非吸烟者和10名非吸烟对照（按年龄，性别和吸烟习惯进行匹配）中抽取静脉血样本。在72小时内分别用标准方法将淋巴细胞培养用于每次测定。在培养时间结束时，收获细胞，染色并编码以进行盲目评分。通过计算每个探针在30个中期中姐妹染色单体交换的数量或通过计数2000个双核细胞中微核的频率来评估DNA物质的交换。此外，测量了有丝分裂和增殖指数。结果：医院的手术室人员暴露于8小时时间加权平均值下，分别为12.8 ppm一氧化二氮和5.3 ppm异氟烷。暴露工人的姐妹染色单体交换的平均（SD）频率显着高于对照组（10.2（1.9）v 7.4（2.4））（p = 0.036），微核比例（微核/ 500个双核细胞）也更高（8.7） （2.9）对6.8（2.5）），但不显着（p = 0.10）。结论：即使暴露于痕量的麻醉气体也可能引起剂量依赖性的遗传损伤。关于微核试验，在平均长期接触废麻醉气体后，未检测到任何致裂潜力。但是，可以检测到人类淋巴细胞中姐妹染色单体交换的频率增加。尽管测得的差异很小，但与每天抽11-20支香烟可比。由于这些发现，微核比例的增加和姐妹染色单体交换的速率可能是长期相关的，需要进一步研究。

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