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首页> 外文期刊>Biomaterials >Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence: a cell morphology study.
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Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence: a cell morphology study.

机译：使用RGDSP-肽序列对钛牙科植入物模型表面进行仿生修饰：细胞形态研究。

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摘要

Surface topography and (bio)chemistry are key factors in determining cell response to an implant. We investigated cell adhesion and spreading patterns of epithelial cells, fibroblasts and osteoblasts on biomimetically modified, smooth and rough titanium surfaces. The RGD bioactive peptide sequence was immobilized via a non-fouling poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) molecular assembly system, which allowed exploitation of specific cell-peptide interactions even in the presence of serum. As control surfaces, bare titanium and bio-inactive surfaces (scrambled RDG and unfunctionalized PLL-g-PEG) were used. Our findings demonstrated that surface topography and chemistry directly influenced the attachment and morphology of all cell types tested. In general, an increase in cell number and more spread cells were observed on bioactive substrates (containing RGD) compared to bio-inactive surfaces. More fibroblasts were present on smooth than on rough topographies, whereas for osteoblasts the opposite tendency was observed. Epithelial cell attachment did not follow any regular pattern. Footprint areas for all cell types were significantly reduced on rough compared to smooth surfaces. Osteoblast attachment and footprint areas increased with increasing RGD-peptide surface density. However, no synergy (interaction) between RGD-peptide surface density and surface topography was observed for osteoblasts neither in terms of attachment nor footprint area.
机译：表面形貌和（生物）化学是确定细胞对植入物反应的关键因素。我们研究了仿生修饰，光滑和粗糙的钛表面上皮细胞，成纤维细胞和成骨细胞的细胞粘附和扩散模式。 RGD生物活性肽序列通过不结垢的聚（L-赖氨酸）-接枝-聚（乙二醇）（PLL-g-PEG）分子组装系统固定，即使在存在条件下也可以利用特定的细胞-肽相互作用血清。作为控制表面，使用了裸钛和无生物活性的表面（扰乱的RDG和未官能化的PLL-g-PEG）。我们的发现表明，表面形貌和化学性质直接影响所测试的所有细胞类型的附着和形态。通常，与具有生物惰性的表面相比，在具有生物活性的底物（包含RGD）上观察到了细胞数量的增加和更多的扩散细胞。光滑表面上的成纤维细胞比粗糙表面上的成纤维细胞多，而成骨细胞则观察到相反的趋势。上皮细胞附着没有遵循任何规则的模式。与光滑表面相比，粗糙表面上所有细胞类型的足迹面积均显着减少。成骨细胞的附着和足迹面积随着RGD肽表面密度的增加而增加。然而，无论从附着力还是在占地面积上都没有观察到成骨细胞的RGD-肽表面密度和表面形貌之间的协同作用（相互作用）。

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