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首页> 外文期刊>Biomaterials >Characterization of non-neuronal elements within fibronectin mats implanted into the damaged adult rat spinal cord
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Characterization of non-neuronal elements within fibronectin mats implanted into the damaged adult rat spinal cord

机译：植入受损成年大鼠脊髓的纤连蛋白垫内的非神经元元素的表征

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Previous studies have shown that mats made from fibronectin (FN) integrate well into spinal cord lesion sites and support extensive axonal growth. Using immunohistochemistry, we have investigated the non-neuronal factors that contribute to these properties. Extensive vascularization was observed in FN mats by 1 week along with heavy inacrophage infiltration by 3 days post-implantation. By 1 week post-implantation, laminin tubules had formed and were associated with axons and p75 immunoreactive Schwann cells. By 4 weeks post-implantation, most axons were associated with Schwarm cell derived myelin. Few oligodendrocytes were present within the mat, even with an increase in the number of oligodendrocyte precursors around the implant site by 7 days post-implantation. Astrocyte proliferation also occurred in the intact tissue, with a prominent glial scar forming around the implant within 4 weeks. However, by 2 months post-implantation astrocytes were present in the FN implant site and were intermingled with the axons. Axonal ingrowth and integration of the FN mats is probably due to the ability of FN mats to support and organize infiltration of Schwarm cells and deposition of laminin. At later time points, myelinated axons remain in the implant site, even after other elements (e.g. macrophages and laminin) have disappeared. Both of these properties are likely to be important in the design of biomaterial bridges for CNS regeneration. (c) 2005 Elsevier Ltd. All rights reserved.
机译：先前的研究表明，由纤连蛋白（FN）制成的垫可以很好地整合到脊髓病变部位，并支持广泛的轴突生长。使用免疫组织化学，我们研究了促成这些特性的非神经元因子。植入后3天，在FN垫上观察到了广泛的血管形成，并在无章鱼大量浸润。植入后1周，层粘连蛋白小管已经形成，并与轴突和p75免疫反应性Schwann细胞相关。植入后4周，大多数轴突与Schwarm细胞衍生的髓磷脂有关。即使在植入后第7天，植入部位周围的少突胶质细胞前体数量增加，垫子中也很少出现少突胶质细胞。星形胶质细胞增殖也发生在完整的组织中，在植入物周围在4周内形成了明显的神经胶质瘢痕。但是，到植入后2个月，星形胶质细胞已经出现在FN植入部位，并与轴突混合在一起。 FN垫的轴突向内生长和整合可能是由于FN垫能够支撑和组织Schwarm细胞的浸润以及层粘连蛋白的沉积。在稍后的时间点，即使在其他元素（例如巨噬细胞和层粘连蛋白）消失后，髓鞘轴突仍保留在植入部位。这两个特性在设计用于CNS再生的生物材料桥时可能很重要。 （c）2005 Elsevier Ltd.保留所有权利。

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