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首页> 外文期刊>Biomaterials >Biomechanical and biochemical characterization of composite tissue-engineered intervertebral discs
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Biomechanical and biochemical characterization of composite tissue-engineered intervertebral discs

机译：复合组织工程化椎间盘的生物力学和生化特性

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Composite tissue-engineered intervertebral tissue was assembled in the shape of cylindrical disks composed of an outer shell of PGA mesh seeded with annulus fibrosus cells with an inner core of nucleus pulposus cells seeded into an alginate get. Samples were implanted subcutaneously in athymic mice and retrieved at time points up to 16 weeks. At all retrieval times, samples maintained shape and contained regions of distinct tissue formation. Histology revealed progressive tissue formation with distinct morphological differences in tissue formation in regions seeded with annulus fibrosus and nucleus pulposus cells. Biochemical analysis indicated that DNA, proteoglycan, and collagen content in tissue-engineered discs increased with time, reaching > 50% of the levels of native tissue by 16 weeks. The exception to this was the collagen content of the nucleus pulposus portion of the implants with were similar to 15% of native values. The equilibrium modulus of tissue-engineered discs was 49.0 +/- 13.2 kPa at 16 weeks, which was between the measured values for the modulus of annulus fibrosus and nucleus pulposus. The hydraulic permeability of tissue-engineered discs was 5.1 +/- 1.7 x 10(-14) m(2)/Pa at 16 weeks, which was between the measured values for the hydraulic permeability of annulus fibrosus and nucleus pulposus. These studies document the feasibility of creating composite tissue-engineered intevertebral disc implants with similar composition and mechanical properties to native tissue. (c) 2005 Elsevier Ltd. All rights reserved.
机译：复合组织工程化的椎间组织被组装成圆柱形圆盘状，该圆盘状由PGA筛网的外壳组成，该外壳上植入了纤维环细胞，髓核细胞的内核被植入了藻酸盐。将样品皮下植入无胸腺小鼠中，并在长达16周的时间点取回。在所有检索时间，样品均保持形状并包含明显的组织形成区域。组织学显示渐进的组织形成，在纤维环和髓核细胞接种的区域中，组织形成具有明显的形态学差异。生化分析表明，组织工程化椎间盘中的DNA，蛋白聚糖和胶原蛋白含量随时间增加，到16周时达到天然组织水平的50％以上。唯一的例外是植入物髓核部分的胶原含量约为原始值的15％。组织工程化椎间盘的平衡模量在16周时为49.0 +/- 13.2 kPa，介于纤维环和髓核模量的测量值之间。组织工程椎间盘的水力渗透率在16周时为5.1 +/- 1.7 x 10（-14）m（2）/ Pa，介于纤维环和髓核的水力渗透率的测量值之间。这些研究证明了创建复合组织工程化椎间盘植入物的可行性，该植入物具有与天然组织相似的组成和机械性能。 （c）2005 Elsevier Ltd.保留所有权利。

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