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首页> 外文期刊>Revue de Medecine Veterinaire >Detection of Mycoplasma synoviae and Mycoplasma galliseptlcum in chickens by immunohistochemical, PGR and culture methods
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Detection of Mycoplasma synoviae and Mycoplasma galliseptlcum in chickens by immunohistochemical, PGR and culture methods

机译：免疫组化，PGR和培养方法检测鸡滑膜支原体和鸡支原体

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摘要

This study aims to determine pathological findings observed in serologically Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) positive chickens and to compare immunohistochemical, PCR and culture methods for the mycoplasmosis diagnosis. Forthat, chnical findings were recorded in 120 broiler chickens from commercial poultry farms serologically MG (n = 27) and MS (n = 93) positive with the rapid slide agglutination test. Following necropsy, tissue samples of sinus, trachea, lung, air sac andknee joint were examined using culture, PCR, histological and immunohistochemical (IHC) techniques. Clinical, anatomical and histological alterations corresponding to a severe acute inflammation were found in 12, 35 and 45 cases respectively. Single MGpositive immunolabelling was observed in 4 cases, mainly in trachea and lungs, strong MS positive labelling, mainly in trachea, air sacs and lungs, in 16 cases whose 6 also exhibited a weak MG staining. The PCR amplification was positive in 14 out of the16 IHC positive cases for MS (211 bp band) and in all IHC positive cases for MG (302 bp band), leading to high agreement scores (97.8% and 100% respectively) whereas MS and MG positive cultures were found in 12 and 3 cases respectively. The agreement scores between IHC and culture and between PCR and culture were 95.7% for the MS detection and 96.3% for the MG detection. Consequently, it was concluded that PCR and IHC methods offer a better sensitivity than the culture methods and may be used in the diagnosis of avian mycoplasmosis.
机译：本研究旨在确定在鸡血清学支原体（MG）和滑膜支原体（MS）阳性鸡中观察到的病理发现，并比较免疫组化，PCR和培养方法对支原体的诊断。为此，在快速家畜凝集试验中，从商业家禽场的120头肉鸡血清学上发现MG（n = 27）和MS（n = 93）阳性。尸检后，使用培养，PCR，组织学和免疫组化（IHC）技术检查窦，气管，肺，气囊和膝关节的组织样本。分别在12、35和45例病例中发现了与严重急性炎症相对应的临床，解剖和组织学改变。在4例中观察到单MG阳性免疫标记，主要在气管和肺中，强MS阳性标记，主要在气管，气囊和肺中，有16例，其中6例也显示MG弱染色。在16例IHC阳性MS（211 bp带）和14例IHC阳性MG中（302 bp带）中14例PCR扩增阳性，导致较高的一致性评分（分别为97.8％和100％）。 MG阳性培养物分别发现12例和3例。 IHC与培养物之间以及PCR与培养物之间的一致性得分在MS检测中为95.7％，在MG检测中为96.3％。因此，得出的结论是，PCR和IHC方法比培养方法具有更高的灵敏度，可用于禽支原体病的诊断。

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