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首页> 外文期刊>Revue de Medecine Veterinaire >Ki67 expression in the cerebellum of dogs with distemper
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Ki67 expression in the cerebellum of dogs with distemper

机译：犬瘟热犬小脑中Ki67的表达

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As the canine distemper virus (CDV) induces demyelination in the central nervous system, partially due to losses of glial cells, the aim of the present study was to determine the Ki67 immunoreactivity in demyelinated regions from cerebellum of dogs with acute and chronic CDV infection. Cerebella from naturally infected dogs (n = 12) were routinely processed for histopathology and the observed lesions were conventionally classified as acute (n = 6) or chronic (n = 6) cases. Immunohistochemical analyses were performed in parallel for detecting the CDV antigen using a mouse anti-CDV monoclonal antibody and for evaluating the proliferation index using a rabbit anti-Ki67 polyclonal antibody. Histological and immunohistochemical findings were compared tohealthy cerebellum controls (n = 6). Contrary to controls, cerebella from infected dogs exhibited demyelinated areas, often moderate to severe, in which the viral presence was confirmed. Regarding the demyelination percentage, no significant difference was found between acute and chronic cases. The glial Ki67 proliferation indexes were roughly similar in the 2 groups of infected dogs and were markedly higher than in controls whereas the mean Ki67 immunopositive glial cell counts were dramatically increased in chronically infected dogs compared to the controls or to the acutely affected dogs. These results show that the glial cell proliferation progresses with the same intensity in both acute and chronic cases, although the total number of Ki67 positivecells is higher in chronically infected dogs, suggesting that compensatory mechanisms for counteracting demyelination.
机译：由于犬瘟热病毒（CDV）引起中枢神经系统脱髓鞘，部分是由于神经胶质细胞的损失，因此，本研究的目的是确定患有急性和慢性CDV感染的狗小脑的脱髓鞘区域的Ki67免疫反应性。常规处理来自自然感染犬的小脑（n = 12）的组织病理学，观察到的病变通常分为急性（n = 6）或慢性（n = 6）病例。并行进行免疫组织化学分析，以使用小鼠抗CDV单克隆抗体检测CDV抗原，并使用兔抗Ki67多克隆抗体评估增殖指数。组织学和免疫组化的发现与健康的小脑对照组进行了比较（n = 6）。与对照相反，来自感染犬的小脑显示出脱髓鞘区域，通常为中度至重度，其中证实存在病毒。关于脱髓鞘百分比，急性和慢性病例之间没有发现显着差异。两组感染狗的神经胶质Ki67增殖指数大致相似，并且显着高于对照组，而与对照组或急性感染的狗相比，慢性感染狗的平均Ki67免疫阳性神经胶质细胞计数显着增加。这些结果表明，尽管在慢性感染的狗中，Ki67阳性细胞的总数更高，但在急性和慢性病例中，神经胶质细胞的增殖以相同的强度进行，这表明抵消脱髓鞘的补偿机制。

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