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首页> 外文期刊>Revue de Medecine Veterinaire >Determination of Culicoides species (Diptera -- Ceratopogonidae) as suspect vectors of Epizootic Haemorrhagic Disease and Blue-tongue viruses in southern and western Anatolia by RT-PCR
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Determination of Culicoides species (Diptera -- Ceratopogonidae) as suspect vectors of Epizootic Haemorrhagic Disease and Blue-tongue viruses in southern and western Anatolia by RT-PCR

机译：通过RT-PCR测定作为安那托利亚南部和西部流行性出血病和蓝舌病毒的可疑媒介的库蚊（Diptera-Ceratopogonidae）

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摘要

This study was performed to detect genomes of the Epizootic Haemorrhagic Disease Virus (EHDV) and Bluetongue virus (BTV) in Culicoides species present in various areas from Anatolia. Culicoides samples were caught using Onderstepoort-type light trapsin 18 districts from 7 Provinces (Antalya, Mugla, Aydm, Izmir, Manisa, Bahkesir and Canakkale) and the presence of viral genomes was investigated by RT-PCR using specific primer pairs (EHDV/S3 and BTV/S7, respectively). Out of the 13 Culicoides species collected, C. imicola complex was predominant following by Culicoides sp, C. nubeculosus, C. schultzei, C. circumscriptus and C. longipennis. The BTV genome was not evidenced in any diptera species whereas the presence of EHDV was detected in 9 species (Cimicola, C. circumscriptus, C. festivi-pennis, C. gejgelensis, C. longipennis, C. nubeculosus, C. obsoletus, C. pu-licaris and Culicoides sp.) depending for the localisation of the collecting centers. The viral genome was evidenced in C. pulicaris and C. imicola complexes, in C. festivipennis, C. gejgelensis, C. longipennis and Culicoides sp. caught in Mugla, in C. imicola, C. longipennis and C. nubeculosus caught in Izmir and in C. obsoletus and C. circumscriptus collected in the Canakkale province. These results show that all of the Culicoides species, from which the viral genome was detected by RT-PCR for the first time in Turkey, were potential EHDV vectors.
机译：进行这项研究的目的是检测安纳托利亚不同地区存在的库库克物种中的流行性出血性疾病病毒（EHDV）和蓝舌病毒（BTV）的基因组。使用Onderstepoort型光阱在来自7个省（安塔利亚，穆拉，阿伊德姆，伊兹密尔，马尼萨，巴克赛尔和恰纳卡莱）的18个区中捕获了库卡虫样品，并使用特定引物对（EHDV / S3）通过RT-PCR研究了病毒基因组的存在和BTV / S7）。在所收集的13个库里科物种中，C。imicola复合体占主导地位，其次是库库科物种，库氏梭菌，schultzei库尔奇，环线虫和长鳍金龟。 BTV基因组在任何物种中均未得到证实，而在9种物种中发现了EHDV（Cimicola，C。circumscriptus，C。festivi-pennis，C。gejgelensis，C。longipennis，C。nubeculosus，C。obsoletus，C。 （例如pu-licaris和Culicoides sp。），具体取决于收集中心的位置。病毒基因组在C. pulicaris和C. imicola复合体，C。festivipennis，C。gejgelensis，C。longipennis和Culicoides sp。中得到了证实。捕获在穆拉，伊米科拉毛虫，longipennis和C. nubeculosus中，捕获于伊兹密尔，恰纳卡莱省收集的废叶梭子鱼和外接角梭菌。这些结果表明，在土耳其首次通过RT-PCR从中检测到病毒基因组的所有Culicoides物种都是潜在的EHDV载体。

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