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首页> 外文期刊>Revue de Medecine Veterinaire >Detection, genotyping, and antigen distribution of bovine viral diarrhoea virus in archival formalin fixed paraffin embedded tissues.
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Detection, genotyping, and antigen distribution of bovine viral diarrhoea virus in archival formalin fixed paraffin embedded tissues.

机译：档案福尔马林固定石蜡包埋组织中牛病毒性腹泻病毒的检测，基因分型和抗原分布。

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摘要

This report identifies and genotypes the bovine viral diarrhoea virus (BVDV) in up to 20 years old archival formalin fixed paraffin embedded (FFPE) tissues of bovine foetuses and calves. Samples from 6 presumably BVDV aborted foetuses and 3 calves diagnosed with mucosal disease were tested by immunohistochemistry (IHC), RT-PCR and a one-step multiplex real time RT-PCR for genotyping. Viral RNA was detected in a 20 years old sample from an aborted foetus at which multiple foci of BVDV viral antigen was localized by IHC in the pulmonary tissue. All three calves with mucosal disease were also RT-PCR positive and BVDV-1 was typed using the one-step multiplex real time RT-PCR. The BVDV antigen distribution was demonstrated in the cerebral cortex (in neurons and their processes and in some glial cells), lung, kidneys, oesophagus, tongue, buccal mucosa and ear pinna of one calve affected with mucosal disease. The highest intensity of the antigen signals were seen in the stratum basale and stratum spinosum of the mucosa of the upper digestive tract tissues. The purpose of this study is to further elucidate the diagnosis and genotyping of BVDV infection with respect to prospective and retrospective identification of the virus in routinely processed and stored tissues for histological evaluation.
机译：这份报告鉴定了牛胎儿和牛犊长达20岁的福尔马林固定石蜡包埋（FFPE）组织中的牛病毒性腹泻病毒（BVDV），并对其进行了基因分型。通过免疫组织化学（IHC），RT-PCR和一步多路实时实时RT-PCR对6名大概是BVDV流产的胎儿和3例诊断为粘膜病的小牛的样本进行基因分型。在流产胎儿的20岁样本中检测到病毒RNA，IHC在肺组织中定位了BVDV病毒抗原的多个病灶。所有三只患有粘膜病的小牛也均为RT-PCR阳性，并使用一步多路实时RT-PCR对BVDV-1进行分型。 BVDV抗原分布在一只患有粘膜病的小牛的大脑皮层（在神经元及其过程以及某些神经胶质细胞中），肺，肾，食道，舌头，颊粘膜和耳廓中得到证实。在上消化道组织的粘膜的基底层和棘层中，抗原信号的强度最高。这项研究的目的是进一步阐明BVDV感染的诊断和基因分型，涉及在常规处理和储存的组织中对病毒进行前瞻性和回顾性鉴定以进行组织学评估。

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