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首页> 外文期刊>Biomaterials >Biomaterial-mediated retroviral gene transfer using self-assembled monolayers.
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Biomaterial-mediated retroviral gene transfer using self-assembled monolayers.

机译：使用自组装单层生物材料介导的逆转录病毒基因转移。

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Biomaterial-mediated gene delivery has recently emerged as a promising alternative to conventional gene transfer technologies that focus on direct delivery of viral vectors or DNA-polymer/matrix complexes. However, biomaterial-based strategies have primarily targeted transient gene expression vehicles, including plasmid DNA and adenovirus particles. This study expands on this work by characterizing biomaterial properties conducive to the surface immobilization of retroviral particles and subsequent transduction of mammalian cells at the cell-material interface. Self-assembled monolayers (SAMs) of functionally-terminated alkanethiols on gold were used to establish biomaterial surfaces of defined chemical composition. Gene transfer was observed to be greater than 90% on NH(2)-terminated surfaces, approximately 50% on COOH-functionalized surfaces, and undetectable on CH(3)-terminated SAMs, similar to controls of tissue culture-treated polystyrene. Gene delivery via the NH(2)-SAM was further characterized as a function of retrovirus coating time, virus concentration, and cell seeding density. Finally, SAM-mediated gene delivery was comparable to fibronectin- and poly-l-lysine-based methods for gene transfer. This work is significant to establishing safe and effective gene therapy strategies, developing efficient methods for gene delivery, and supporting recent progress in the field of biomaterial-mediated gene transfer.
机译：最近，生物材料介导的基因传递已成为传统基因转移技术的有前途的替代方法，后者专注于直接传递病毒载体或DNA-聚合物/基质复合物。但是，基于生物材料的策略主要针对瞬时基因表达载体，包括质粒DNA和腺病毒颗粒。这项研究通过表征有助于逆转录病毒颗粒表面固定和随后在细胞-材料界面上转导哺乳动物细胞的生物材料特性，扩展了这项工作。功能终止的链烷硫醇在金上的自组装单分子层（SAMs）用于建立具有确定化学组成的生物材料表面。观察到基因转移在NH（2）终止的表面上大于90％，在COOH官能化的表面上大于50％，在CH（3）终止的SAMs上检测不到，类似于组织培养物处理的聚苯乙烯的对照。通过NH（2）-SAM的基因传递进一步表征为逆转录病毒包被时间，病毒浓度和细胞接种密度的函数。最后，SAM介导的基因传递与基于纤连蛋白和聚-1-赖氨酸的基因传递方法相当。这项工作对于建立安全有效的基因治疗策略，开发有效的基因传递方法以及支持生物材料介导的基因转移领域的最新进展具有重要意义。

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