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首页> 外文期刊>Biomaterials >Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo.
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Assembly of collagen-binding peptide with collagen as a bioactive scaffold for osteogenesis in vitro and in vivo.

机译：胶原蛋白结合肽与胶原蛋白作为生物活性支架的组装，用于体外和体内成骨。

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摘要

Bioactive scaffolds inducing cell adhesion, differentiation have been premise for optimal formation of target tissue. Collagen has been employed as a tissue regenerative scaffold especially for bone regeneration and has been chemically surface-modified to present bioactivity. Herein, we show that peptide, denoted as collagen-binding motif (CBM, GLRSKSKKFRRPDIQYPDATDEDITSHM) identified from osteopontin (OPN) protein, was able to specifically bind collagen without chemical conjugation, while presenting apatite forming capability in vitro and in vivo. Collagen surface alone was not able to induce noticeable apatite nucleation however, mineralization was evident when assembled with CBM peptide, implying that the collagen-CBM assembly played a pivotal role in biomineralization. In vivo result further demonstrated that the CBM peptide in complex with material was able to induce bone formation by helping mineralization in the bone defect. Taken together, the CBM peptide herein and its assembly with collagen can be applied as an inducer of biomineralization as well as a bioactive scaffold for bone regeneration.
机译：诱导细胞粘附，分化的生物活性支架已成为靶组织最佳形成的前提。胶原蛋白已被用作组织再生支架，特别是用于骨再生，并且已经进行了化学表面修饰以表现出生物活性。本文中，我们显示了从骨桥蛋白（OPN）蛋白鉴定出的，被称为胶原蛋白结合基序（CBM，GLRSKSKKFRRPDIQYPDATDEDITSHM）的肽能够特异性结合胶原蛋白而无需化学偶联，同时在体内和体外呈现出磷灰石形成能力。单独的胶原蛋白表面不能诱导明显的磷灰石成核，但是，当与CBM肽组装时，矿化作用是明显的，这意味着胶原蛋白-CBM组装物在生物矿化中起关键作用。体内结果进一步证明，与材料复合的CBM肽能够通过帮助骨缺损矿化来诱导骨形成。综上所述，本文的CBM肽及其与胶原的组装可以用作生物矿化的诱导剂以及用于骨再生的生物活性支架。

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