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首页> 外文期刊>Cellular Signalling >Effect of pregnancy on PDE4 cAMP-specific phosphodiesterase messenger ribonucleic acid expression in human myometrium.
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Effect of pregnancy on PDE4 cAMP-specific phosphodiesterase messenger ribonucleic acid expression in human myometrium.

机译：妊娠对人子宫肌层中PDE4 cAMP特异性磷酸二酯酶信使核糖核酸表达的影响。

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摘要

In light of the important role of the second messengers cAMP and cGMP in the mechanism of relaxation in the human myometrium, specific regulation of the phosphodiesterase (PDE) enzymatic system responsible for cyclic nucleotide inactivation is essential. We previously identified in the human myometrium PDE4 cAMP-specific PDE as by far the most abundant isoform. Here we have studied the expression patterns of mRNAs for the four cloned human PDE4 genes in the myometria of pregnant and non-pregnant women. Concurrent expression of the PDE4A, 4B, 4C and 4D genes is demonstrated. We found that the PDE4D transcripts are the most prominently expressed. PDE4A and PDE4B mRNAs also are markedly abundant, whereas lower expression is observed for PDE4C mRNAs. Interestingly, we showed that transcripts of PDE4B2 are more abundant in the myometria of pregnant women than in non-pregnant women, whereas no difference between the two tissues was detected for PDE4A, 4C and 4D mRNAs. Cultured human myometrial cells, which present a high level of PDE4 activity and express the four PDE4 mRNA subtypes, provide us with an appropriate model to further evaluate whether the level of expression of the PDE 4B2 mRNA subtype is under hormonal regulation.
机译：鉴于第二信使cAMP和cGMP在人子宫肌层松弛机制中的重要作用，对负责环核苷酸失活的磷酸二酯酶（PDE）酶系统的特定调节至关重要。我们先前在人子宫肌层中发现PDE4 cAMP特异性PDE是迄今为止最丰富的同工型。在这里，我们研究了孕妇和非孕妇的子宫肌层中四个克隆的人PDE4基因的mRNA表达模式。证明了PDE4A，4B，4C和4D基因的同时表达。我们发现PDE4D成绩单表达最突出。 PDE4A和PDE4B mRNA也明显丰富，而PDE4C mRNA则表达较低。有趣的是，我们显示孕妇的子宫肌层中PDE4B2的转录物比未怀孕的妇女中的丰富，而在这两个组织之间未检测到PDE4A，4C和4D mRNA的差异。具有高水平PDE4活性并表达四种PDE4 mRNA亚型的培养的人子宫肌细胞，为我们提供了合适的模型，以进一步评估PDE 4B2 mRNA亚型的表达水平是否处于激素调节之下。

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