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首页> 外文期刊>Oncology: International Journal of Cancer Research and Treatment >Mucin mRNA expression in lung adenocarcinoma cell lines and tissues.
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Mucin mRNA expression in lung adenocarcinoma cell lines and tissues.

机译：肺腺癌细胞系和组织中黏蛋白mRNA表达。

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摘要

The expression pattern of mucin genes was studied in 7 lung adenocarcinoma cell lines (CL1, CL2, CL3, NCL2, PC9, PC13, PC14) and 12 lung adenocarcinoma tissues. CL1 and PC13 are poorly differentiated cell lines with low mucin glycoprotein production. The other 5 cell lines are well differentiated and produce a higher amount of mucins. Total RNA was extracted from these cell lines. Northern blot analysis was performed by hybridization with specific antisense oligonucleotide probes recognizing mucin-specific tandem repeats of 4 mucin genes (MUC1, MUC2, MUC3, MUC4). RT-PCR was carried out to amplify the 3' and 5' nonrepetitive coding regions of MUC1 and the 5' nonrepetitive coding region of MUC2. All these cell lines expressed MUC1, MUC2, and MUC4 mRNA but in variable mounts. The poorly differentiated cell lines (CL1 and PC13) had a relatively low level of expression of MUC1, MUC2, MUC3 and MUC4. RT-PCR, with primers amplifying the MUC1 nonrepetitive coding region 5' end, 293 bp, and the 3' end, 522 bp, as well as the MUC2 nonrepetitive 5' coding region, 308 bp, revealed the presence of MUC1 and MUC2 mRNA in all the cell lines. Sequence analysis of the PCR products were very homologous, similar to previously published MUC1 and MUC2 cDNA sequences. The expression pattern of mucin genes is consistent with that of mucin glycoproteins as studied using biochemical and immunological methods. Northern blotting and RT-PCR analysis in 12 lung adenocarcinoma tissues with various grades of differentiation (6 poorly differentiated adenocarcinomas and 6 moderately to well-differentiated adenocarcinomas) showed heterogeneous expression of the 4 mucin genes in tissues without clear correlation with the differentiation grade. Therefore the clinical implications of the differential expression of the mucin genes need further investigation.
机译：研究了粘蛋白基因在7种肺腺癌细胞系（CL1，CL2，CL3，NCL2，PC9，PC13，PC14）和12种肺腺癌组织中的表达模式。 CL1和PC13是低分化的细胞系，粘蛋白糖蛋白生成量低。其他5个细胞系分化良好，并产生更多的粘蛋白。从这些细胞系中提取总RNA。通过与识别4个粘蛋白基因（MUC1，MUC2，MUC3，MUC4）的粘蛋白特异性串联重复序列的特异性反义寡核苷酸探针杂交进行Northern印迹分析。进行RT-PCR以扩增MUC1的3'和5'非重复编码区和MUC2的5'非重复编码区。所有这些细胞系均表达MUC1，MUC2和MUC4 mRNA，但坐骑可变。低分化细胞系（CL1和PC13）的MUC1，MUC2，MUC3和MUC4的表达水平相对较低。使用引物扩增MUC1非重复编码区5'端293 bp和3'末端522 bp以及MUC2非重复5'编码区308 bp的RT-PCR揭示了MUC1和MUC2 mRNA的存在在所有细胞系中PCR产物的序列分析非常同源，类似于先前发表的MUC1和MUC2 cDNA序列。粘蛋白基因的表达模式与使用生化和免疫学方法研究的粘蛋白糖蛋白的表达模式一致。 Northern印迹和RT-PCR分析在12种不同分化程度的肺腺癌组织中（6种低分化腺癌和6种中分化至高分化腺癌）显示了4种粘蛋白基因在组织中的异质表达，与分化程度没有明显相关性。因此，粘蛋白基因差异表达的临床意义需要进一步研究。

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