• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Signalling >Circadian clock regulation of the bimodal rhythm of cyclic AMP in wild-type Euglena.
【24h】

Circadian clock regulation of the bimodal rhythm of cyclic AMP in wild-type Euglena.

机译：野生型裸藻（Euglena）中环AMP双峰节律的昼夜节律调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cell-cycle traverse is associated with fluctuations in the cellular content of cAMP; artificial alterations of these levels phase-shift cell division in free-running cultures of achlorophyllous Euglena maintained in constant darkness (DD). The phase shifts observed, however, are only transient: the cell division rhythm rephases to that of unperturbed controls. This implies that the second messenger functions downstream of the circadian oscillator. Further, the level of cAMP is known to indicate carbon nutrient status and the competency of cells to traverse various restriction points in the cell cycle of other eukaryotes. We wished to determine the profile of cAMP content in free-running, dividing and non-dividing cultures of green, wild-type cells, which survive well during prolonged growth arrest. We monitored cAMP content in photoautothropic cultures of E. gracilis (strain Z) at 25 degrees C under either an entraining light-dark cycle comprising 12 h of light and 12 h of darkness (LD:12,12) or free-running (LD:1/2,1/2) regimes. cAMP content in rhythmically dividing, light-phased or free-running cells exhibited bimodality [peaks at CT (circadian time) 9-14 and CT 19-22). Expression of cAMP content on a per milligram total cellular protein basis caused the day trough (CT 1-3) to be even more distinct. Non-dividing, free-running, photoautotrophic cultures displayed a similarly phased bimodality in cAMP content. These findings in wild-type Euglena confirm that the bimodal rhythm of cAMP content is regulated by the circadian oscillator that underlies division rhythmicity but is not dependent on the cell division cycle. We will now determine the effect of the fluctuating cAMP levels on the phosphorylation status and activity of cell-cycle regulatory proteins.
机译：细胞周期遍历与cAMP细胞含量的波动有关。在自由运行的无叶绿藻Euglena培养物中，这些水平的相移细胞分裂的人工改变保持在恒定的黑暗（DD）中。但是，观察到的相移只是暂时的：细胞分裂的节奏会重新调整为不受干扰的对照的节奏。这意味着第二信使在昼夜节律振荡器的下游起作用。此外，已知cAMP的水平可指示碳营养状况以及细胞穿越其他真核生物细胞周期中各种限制点的能力。我们希望确定在绿色，野生型细胞的自由运行，分裂和非分裂培养物中的cAMP含量概况，这些培养物在长时间的生长停滞中可以很好地存活。我们在包括12 h光照和12 h黑暗（LD：12,12）或自由运行（LD）的引诱的明暗循环下，在25摄氏度下监测了细粒肠球菌（Z菌株）的光自然人类培养中的cAMP含量。 ：1 / 2,1 / 2）制度。在有节奏地分裂，光相位或自由运行的细胞中，cAMP含量表现出双峰性（在CT（昼夜节律时间）9-14和CT 19-22时达到峰值）。以每毫克总细胞蛋白为基础的cAMP含量表达导致日谷（CT 1-3）更加明显。非分隔，自由运行，光合自养培养物在cAMP含量方面表现出相似的阶段性双峰性。这些在野生型Euglena中的发现证实，cAMP含量的双峰节律是由昼夜节律调节的，该节律是分裂节律的基础，但不依赖于细胞分裂周期。现在，我们将确定波动的cAMP水平对细胞周期调控蛋白的磷酸化状态和活性的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号