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首页> 外文期刊>Reproductive sciences >Human chorionic gonadotropin (hCG) in the secretome of cultured embryos: Hyperglycosylated hCG and hCG-free beta subunit are potential markers for infertility management and treatment
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Human chorionic gonadotropin (hCG) in the secretome of cultured embryos: Hyperglycosylated hCG and hCG-free beta subunit are potential markers for infertility management and treatment

机译：培养的胚胎的分泌基因组中的人绒毛膜促性腺激素（hCG）：高糖基化的hCG和不含hCG的β亚基是不孕症管理和治疗的潜在标志物

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Human chorionic gonadotropin (hCG) is produced by trophoblast cells throughout pregnancy, and gene expression studies have indicated that hCG-beta subunit (hCGβ) expression is active at the 2 blastomere stage. Here, we investigated the qualitative hCG output of developing embryos in culture and hCG isoforms expressed in the secretome as a novel sensitive method for detecting hCG. Culture media was collected from the culture plates of 118 embryos in culture (including controls and embryos at different stages of culture) from 16 patients undergoing routine fertility treatment. The hCGβ was detectable in media from 2 pronuclear (2PN) stage embryos through to the blastocyst stage. The hCGβ was absent in 1PN and arrested embryos as well as all media controls. Prior to hatching, hyperglycosylated hCG (hCGh) was observed selectively in 3PN embryos, but after hatching, along with hCG, became the dominant hCG molecule observed. We have reported at the 2PN stage the earliest evidence of hCGβ expression in embryos. There is a suggestion this may be indicative of quality in early embryos, and hCGh seen at the pronuclear stage may suggest triploid abnormality. The dominance of hCG, and hCGh expression, seen after blastocyst hatching may be indicative of potential implantation success. Thus, hCG isoforms have potential roles as biomarkers of embryo viability for embryo/blastocyst transfer.
机译：人绒毛膜促性腺激素（hCG）在整个怀孕期间都由滋养层细胞产生，并且基因表达研究表明hCG-β亚基（hCGβ）的表达在两个卵裂球阶段均具有活性。在这里，我们调查了文化中发育中的胚胎的定性hCG输出和在分泌组中表达的hCG亚型，作为检测hCG的新型灵敏方法。从118例接受常规生育治疗的患者的培养物中的培养基（包括对照和处于不同培养阶段的胚胎）的培养板中收集培养基。在从2个前核（2PN）阶段胚胎到胚泡阶段的培养基中都可以检测到hCGβ。 hCGβ在1PN中不存在，并且被捕的胚胎以及所有培养基对照。孵化前，在3PN胚胎中选择性观察到了高糖基化的hCG（hCGh），但是孵化后，与hCG一起成为观察到的主要hCG分子。我们已经报道了在2PN阶段hCGβ在胚胎中表达的最早证据。有迹象表明，这可能表明早期胚胎的质量，并且在前核阶段观察到的hCGh可能表明三倍体异常。胚泡孵化后看到的hCG和hCGh表达优势可能表明潜在的植入成功。因此，hCG同工型具有潜在的作用，作为胚胎存活能力的生物标志物，用于胚胎/胚泡转移。

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