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首页> 外文期刊>Reproductive toxicology >Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function.
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Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function.

机译：荧光探针和流式细胞仪评估大鼠精子的完整性和线粒体功能。

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摘要

Fluorescent assessment of cellular integrity and mitochondrial function by flow cytometry can provide a rapid and precise means of determining the functional status of large numbers of spermatozoa. In the present study, rat sperm viability was assessed with SYBR-14 and PI and sperm mitochondria were differentially labeled with JC-1. Sperm samples of variable viability were prepared using varying proportions of fresh and frozen spermatozoa. SYBR-14 stained sperm correlated well with expected sperm viability (r = 0.98). Motile sperm stained with JC-1 appeared orange in the midpiece indicating a high mitochondrial membrane potential whereas immotile sperm with a low membrane potential stained green. The percentage of spermatozoa staining orange was highly correlated (r = 0.99) with expected sperm viability. Flow cytometry using specific fluorescent probes is a useful technique for detecting changes in rat sperm plasma membrane integrity and mitochondrial function in large numbers of spermatozoa.
机译：通过流式细胞术对细胞完整性和线粒体功能进行荧光评估可以提供一种快速而精确的方法来测定大量精子的功能状态。在本研究中，用SYBR-14评估了大鼠的精子生存力，并用JC-1差异标记了PI和精子线粒体。使用不同比例的新鲜和冷冻精子制备可变活力的精子样品。 SYBR-14染色的精子与预期的精子生存能力密切相关（r = 0.98）。用JC-1染色的活动精子在中段出现橙色，表明线粒体膜电位高，而具有较低膜电位的活动精子则染成绿色。精子染橙色的百分比与预期的精子生存能力高度相关（r = 0.99）。使用特定荧光探针的流式细胞仪是检测大量精子中大鼠精子质膜完整性和线粒体功能变化的有用技术。

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