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首页> 外文期刊>Reproductive toxicology >An evaluation of the chick cardiomyocyte micromass system for identification of teratogens in a blind trial.
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An evaluation of the chick cardiomyocyte micromass system for identification of teratogens in a blind trial.

机译：在盲法试验中对雏鸡心肌细胞微质量系统鉴定致畸物的评估。

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摘要

The chick micromass culture system has advantages over the validated rat system - ready availability and non-culling of the donor parent - but needs to give comparable results. This study confirmed comparability and the ability to extend the system to cover cardiac effects. It was also compared with the validated embryonic stem cell cardiomyocyte model. A teratogen and paired non-teratogen with known in vivo effects were used. Differential effects were measured via changes in cell protein content, cell viability (resazurin reduction and neutral red uptake), and cell contractility. Results showed that teratogens [L-ethionine, 5-fluorouracil and sulphisoxazole] could be distinguished from non-teratogens [DL-methionine, 6-methyluracil and sulphanilamide respectively]. Dichloroacetone and dichloropropanol affected embryonic stem cells but not the micromass; dichloropropanol had a greater effect than dichloroacetone. This approach revealed differential effects on contractility independent of effects on activity/viability, whilst the total cell protein remained unchanged. We suggest that pre-validation of this system should be examined.
机译：小鸡微质量培养系统具有优于已验证的大鼠系统的优势-易于获得且未剔除供体亲本-但需要给出可比的结果。这项研究证实了可比性以及扩展该系统以覆盖心脏效应的能力。还与经过验证的胚胎干细胞心肌细胞模型进行了比较。使用具有已知体内效应的致畸剂和成对的非致畸剂。通过改变细胞蛋白含量，细胞活力（刃天青素减少和中性红摄取）和细胞收缩力来测量差异作用。结果表明，致畸剂[L-乙硫氨酸，5-氟尿嘧啶和磺胺异恶唑]可与非致畸剂[分别为DL-甲硫氨酸，6-甲基尿嘧啶和磺胺]。二氯丙酮和二氯丙醇会影响胚胎干细胞，但不会影响其微量。二氯丙醇比二氯丙酮具有更大的作用。该方法揭示了对收缩力的不同影响，而与对活性/生存力的影响无关，而总细胞蛋白保持不变。我们建议应检查此系统的预验证。

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